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Title: Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes

Abstract

The serotonergic system in the central nervous system (CNS) has been implicated in many physiological functions. The objectives of this study are: (1) to develop a new photoaffinity probe that can be used to identify the protein associated with the substrate binding site of the 5-HT uptake carrier, (2) to investigate the existence of different conformational states of the 5-HT carrier, (3) to solubilize the 5-HT/sub 1a/ receptor proteins from bovine hippocampus, and (4) to identify the 5-HT/sub 1a/ receptor proteins using a new photoaffinity probe, i.e., 1-(2-(4-aminophenyl)ethyl)4-(3-trifluoromethylphenyl)piperazine ((/sup 3/H)p-azido-PAPP), with high affinity for 5-HT/sub 1a/ receptor.

Authors:
Publication Date:
OSTI Identifier:
6439353
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CENTRAL NERVOUS SYSTEM; BIOCHEMISTRY; SEROTONIN; RECEPTORS; UPTAKE; AFFINITY; BIOCHEMICAL REACTION KINETICS; BRAIN; CATTLE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BODY; CHEMISTRY; DOMESTIC ANIMALS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; PYRROLES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; RUMINANTS; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lee, J D. Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes. United States: N. p., 1986. Web.
Lee, J D. Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes. United States.
Lee, J D. 1986. "Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes". United States.
@article{osti_6439353,
title = {Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes},
author = {Lee, J D},
abstractNote = {The serotonergic system in the central nervous system (CNS) has been implicated in many physiological functions. The objectives of this study are: (1) to develop a new photoaffinity probe that can be used to identify the protein associated with the substrate binding site of the 5-HT uptake carrier, (2) to investigate the existence of different conformational states of the 5-HT carrier, (3) to solubilize the 5-HT/sub 1a/ receptor proteins from bovine hippocampus, and (4) to identify the 5-HT/sub 1a/ receptor proteins using a new photoaffinity probe, i.e., 1-(2-(4-aminophenyl)ethyl)4-(3-trifluoromethylphenyl)piperazine ((/sup 3/H)p-azido-PAPP), with high affinity for 5-HT/sub 1a/ receptor.},
doi = {},
url = {https://www.osti.gov/biblio/6439353}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}

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