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Title: Extinction of Oct-3/4 gene expression in embryonal carcinoma [times] fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region

Abstract

The OCT-3/4 gene provides an excellent model system with which to study the extinction phenomenon in somatic cell hybrids. The molecular mechanism that underlies the extinction of a tissue-specific transcription factor in somatic cell hybrides is evaluated and compared with its down-regulation in retinoic acid treated embryonal carcinoma cells. This study draws a connection between the shutdown of OCT-3/4 expression in retinoic acid (RA)-differentiated embryonal carcinoma (EC) cells and its extinction in hybrid cells. This repression of OCT-3/4 expression is achieved through changes in the methylation status, chromatin structure, and transcriptional activity of the OCT-3/4 upstream regulatory region. 59 refs.

Authors:
; ; ;  [1]
  1. Hebrew Univ., Jerusalem (Israel)
Publication Date:
OSTI Identifier:
6434365
Resource Type:
Journal Article
Journal Name:
Molecular and Cellular Biology; (United States)
Additional Journal Information:
Journal Volume: 13:2; Journal ID: ISSN 0270-7306
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NEOPLASMS; SOMATIC CELLS; CARCINOGENS; GENE OPERONS; GENES; METHYLATION; SOMATIC MUTATIONS; ANIMAL CELLS; CHEMICAL REACTIONS; DISEASES; MUTATIONS; 550200* - Biochemistry

Citation Formats

Ben-Shushan, E, Pikarsky, E, Klar, A, and Bergman, Y. Extinction of Oct-3/4 gene expression in embryonal carcinoma [times] fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region. United States: N. p., 1993. Web. doi:10.1128/MCB.13.2.891.
Ben-Shushan, E, Pikarsky, E, Klar, A, & Bergman, Y. Extinction of Oct-3/4 gene expression in embryonal carcinoma [times] fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region. United States. https://doi.org/10.1128/MCB.13.2.891
Ben-Shushan, E, Pikarsky, E, Klar, A, and Bergman, Y. 1993. "Extinction of Oct-3/4 gene expression in embryonal carcinoma [times] fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region". United States. https://doi.org/10.1128/MCB.13.2.891.
@article{osti_6434365,
title = {Extinction of Oct-3/4 gene expression in embryonal carcinoma [times] fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region},
author = {Ben-Shushan, E and Pikarsky, E and Klar, A and Bergman, Y},
abstractNote = {The OCT-3/4 gene provides an excellent model system with which to study the extinction phenomenon in somatic cell hybrids. The molecular mechanism that underlies the extinction of a tissue-specific transcription factor in somatic cell hybrides is evaluated and compared with its down-regulation in retinoic acid treated embryonal carcinoma cells. This study draws a connection between the shutdown of OCT-3/4 expression in retinoic acid (RA)-differentiated embryonal carcinoma (EC) cells and its extinction in hybrid cells. This repression of OCT-3/4 expression is achieved through changes in the methylation status, chromatin structure, and transcriptional activity of the OCT-3/4 upstream regulatory region. 59 refs.},
doi = {10.1128/MCB.13.2.891},
url = {https://www.osti.gov/biblio/6434365}, journal = {Molecular and Cellular Biology; (United States)},
issn = {0270-7306},
number = ,
volume = 13:2,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1993},
month = {Mon Feb 01 00:00:00 EST 1993}
}