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Title: Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats

Abstract

Studies were conducted in rats to determine the effect of dietary selenium (Se) concentration on hepatic glutathione concentrations and enzyme activities associated with the maintenance of the cellular glutathione status. Male rats were fed 0.1, 3.0, or 6.0 ppm Se as Na/sub 2/SeO/sub 3/ for 2, 4, or 6 weeks at which time they were killed and analyses were performed. Both 3.0 and 6.0 ppm Se caused a significant dose-dependent increase in hepatic-reduced glutathione (GSH) by 4 weeks of feeding compared to 0.1 ppm Se. The increase in GSH was preceded by significant, dose-dependent increases in oxidized glutathione (GSSG) as well as the GSSG to GSH ratio. Increases in GSSG and the GSSG to GSH ratio as well as in glutathione reductase and glucose-6-phosphate dehydrogenase activities were observed by 2 weeks of high Se feeding. The current findings substantiate previous results demonstrating effects of high Se on hepatic glutathione concentrations and further suggest that increased cellular GSSG concentrations or the GSSG to GSH ratio caused by 3.0 and 6.0 ppm dietary Se signals for adaptive changes in hepatic glutathione metabolism.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison
OSTI Identifier:
6425552
Resource Type:
Journal Article
Journal Name:
Proc. Soc. Exp. Biol. Med.; (United States)
Additional Journal Information:
Journal Volume: 180:2; Journal Issue: 2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; GLUTATHIONE; BIOCHEMICAL REACTION KINETICS; SELENIUM; BIOLOGICAL EFFECTS; DIET; DOSE-RESPONSE RELATIONSHIPS; ENZYME ACTIVITY; EXPERIMENTAL DATA; INGESTION; LIVER; METABOLISM; OXIDOREDUCTASES; RATS; REDOX REACTIONS; ANIMALS; BODY; CHEMICAL REACTIONS; DATA; DIGESTIVE SYSTEM; DRUGS; ELEMENTS; ENZYMES; GLANDS; INFORMATION; INTAKE; KINETICS; MAMMALS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; RODENTS; SEMIMETALS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 550200 - Biochemistry

Citation Formats

LeBoeuf, R A, Zentner, K L, and Hoekstra, W G. Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats. United States: N. p., 1985. Web. doi:10.3181/00379727-180-42187.
LeBoeuf, R A, Zentner, K L, & Hoekstra, W G. Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats. United States. https://doi.org/10.3181/00379727-180-42187
LeBoeuf, R A, Zentner, K L, and Hoekstra, W G. 1985. "Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats". United States. https://doi.org/10.3181/00379727-180-42187.
@article{osti_6425552,
title = {Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats},
author = {LeBoeuf, R A and Zentner, K L and Hoekstra, W G},
abstractNote = {Studies were conducted in rats to determine the effect of dietary selenium (Se) concentration on hepatic glutathione concentrations and enzyme activities associated with the maintenance of the cellular glutathione status. Male rats were fed 0.1, 3.0, or 6.0 ppm Se as Na/sub 2/SeO/sub 3/ for 2, 4, or 6 weeks at which time they were killed and analyses were performed. Both 3.0 and 6.0 ppm Se caused a significant dose-dependent increase in hepatic-reduced glutathione (GSH) by 4 weeks of feeding compared to 0.1 ppm Se. The increase in GSH was preceded by significant, dose-dependent increases in oxidized glutathione (GSSG) as well as the GSSG to GSH ratio. Increases in GSSG and the GSSG to GSH ratio as well as in glutathione reductase and glucose-6-phosphate dehydrogenase activities were observed by 2 weeks of high Se feeding. The current findings substantiate previous results demonstrating effects of high Se on hepatic glutathione concentrations and further suggest that increased cellular GSSG concentrations or the GSSG to GSH ratio caused by 3.0 and 6.0 ppm dietary Se signals for adaptive changes in hepatic glutathione metabolism.},
doi = {10.3181/00379727-180-42187},
url = {https://www.osti.gov/biblio/6425552}, journal = {Proc. Soc. Exp. Biol. Med.; (United States)},
number = 2,
volume = 180:2,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1985},
month = {Fri Nov 01 00:00:00 EST 1985}
}