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Title: Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells

Abstract

The addition of bradykinin to NG108-15 cells results in a transient hyperpolarization followed by prolonged cell depolarization. Injection of inositol 1,4,5-trisphosphate or CaS into the cytoplasm of NG108-15 cells also elicits cell hyperpolarization followed by depolarization. Tetraethylammonium ions inhibit the hyperpolarizing response of cells to bradykinin or inositol 1,4,5-trisphosphate. Thus, the hyperpolarizing phase of the cell response may be due to inositol 1,4,5-trisphosphate-dependent release of stored UVCa-labelled CaS into the cytoplasm, which activates CaS -dependent K channels. The depolarizing phase of the cell response to bradykinin is due largely to inhibition of M channels, thereby decreasing the rate of K efflux from cells and, to a lesser extent, to activation of CaS -dependent ion channels and CaS channels. In contrast, injection of inositol 1,4,5-trisphosphate or CaS into the cytosol did not alter M channel activity. Incubation of NG108-15 cells with pertussis toxin inhibits bradykinin-dependent cell hyperpolarization and depolarization. Bradykinin stimulates low K/sub m/ GTPase activity and inhibits adenylate cyclase in NG108-15 membrane preparations but not in membranes prepared from cells treated with pertussis toxin. These results show that (bradykinin-receptor) complexes interact with N/sub o/ or N/sub i/ and suggest that N/sub o/ and/or N/sub i/ mediate the transduction of signalsmore » from bradykinin receptors to phospholipase C and adenylate cyclase.« less

Authors:
; ; ;
Publication Date:
Research Org.:
National Institutes of Health (NIH), Bethesda, MD (United States)
OSTI Identifier:
6415710
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 83:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CALCIUM 45; UPTAKE; MEMBRANE TRANSPORT; CHEMICAL ACTIVATION; TUMOR CELLS; ELECTRIC POTENTIAL; BIOELECTRICITY; BRADYKININ; CALCIUM CHLORIDES; CYCLASES; INOSITOLS; TEAB; TOXINS; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METAL COMPOUNDS; AMINES; AMMONIUM COMPOUNDS; ANIMAL CELLS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BROMIDES; BROMINE COMPOUNDS; CALCIUM COMPOUNDS; CALCIUM HALIDES; CALCIUM ISOTOPES; CARBOHYDRATES; CHLORIDES; CHLORINE COMPOUNDS; DAYS LIVING RADIOISOTOPES; ELECTRICITY; ENZYMES; EVEN-ODD NUCLEI; HALIDES; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; KININS; LYASES; MATERIALS; MONOSACCHARIDES; NUCLEI; ORGANIC COMPOUNDS; PEPTIDES; POLYPEPTIDES; PROTEINS; QUATERNARY COMPOUNDS; RADIOISOTOPES; SACCHARIDES; TOXIC MATERIALS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Higashida, H, Streaty, R A, Klee, W, and Nirenberg, M. Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells. United States: N. p., 1986. Web. doi:10.1073/pnas.83.4.942.
Higashida, H, Streaty, R A, Klee, W, & Nirenberg, M. Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells. United States. https://doi.org/10.1073/pnas.83.4.942
Higashida, H, Streaty, R A, Klee, W, and Nirenberg, M. 1986. "Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells". United States. https://doi.org/10.1073/pnas.83.4.942.
@article{osti_6415710,
title = {Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells},
author = {Higashida, H and Streaty, R A and Klee, W and Nirenberg, M},
abstractNote = {The addition of bradykinin to NG108-15 cells results in a transient hyperpolarization followed by prolonged cell depolarization. Injection of inositol 1,4,5-trisphosphate or CaS into the cytoplasm of NG108-15 cells also elicits cell hyperpolarization followed by depolarization. Tetraethylammonium ions inhibit the hyperpolarizing response of cells to bradykinin or inositol 1,4,5-trisphosphate. Thus, the hyperpolarizing phase of the cell response may be due to inositol 1,4,5-trisphosphate-dependent release of stored UVCa-labelled CaS into the cytoplasm, which activates CaS -dependent K channels. The depolarizing phase of the cell response to bradykinin is due largely to inhibition of M channels, thereby decreasing the rate of K efflux from cells and, to a lesser extent, to activation of CaS -dependent ion channels and CaS channels. In contrast, injection of inositol 1,4,5-trisphosphate or CaS into the cytosol did not alter M channel activity. Incubation of NG108-15 cells with pertussis toxin inhibits bradykinin-dependent cell hyperpolarization and depolarization. Bradykinin stimulates low K/sub m/ GTPase activity and inhibits adenylate cyclase in NG108-15 membrane preparations but not in membranes prepared from cells treated with pertussis toxin. These results show that (bradykinin-receptor) complexes interact with N/sub o/ or N/sub i/ and suggest that N/sub o/ and/or N/sub i/ mediate the transduction of signals from bradykinin receptors to phospholipase C and adenylate cyclase.},
doi = {10.1073/pnas.83.4.942},
url = {https://www.osti.gov/biblio/6415710}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 83:4,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 1986},
month = {Sat Feb 01 00:00:00 EST 1986}
}