Biosynthesis and secretion of follicle-stimulating hormone beta-subunit from ovine pituitary cultures: effect of 17 beta-estradiol treatment

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · Endocrinology; (United States)

OSTI ID:6414139

Whitfield, G K; Miller, W L

An assay was developed to detect tritium-labeled ovine FSH beta-subunit (( /sup 3/H)oFSH beta) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSH beta (RCM-oFSH beta) in a two-cycle immunoextraction procedure. A discrete species with an apparent mol wt of 21,000 was detected in sodium dodecyl sulfate electrophoretic patterns of immunoextracts from culture medium. This species was identified as RCM-(/sup 3/H)oFSH beta by its comigration with highly purified RCM-oFSH beta, its reduction in culture media after cultures were treated with 17 beta-estradiol, which normally decreases radioimmunoassayable oFSH; and its displacement from the extracting antibodies by excess unlabeled RCM-oFSH beta. The assay was used in a pulse-chase study to determine that (/sup 3/H)oFSH beta is secreted within 1-2 h of its synthesis. Prior treatment of cultures with 17 beta-estradiol did not change this timing of secretion.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 6414139
• Journal Information: Endocrinology; (United States), Vol. 115:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESTRADIOL
BIOLOGICAL EFFECTS
FSH
BIOSYNTHESIS
MOLECULAR WEIGHT
CELL CULTURES
ELECTROPHORESIS
PITUITARY GLAND
TRITIUM COMPOUNDS
BODY
ENDOCRINE GLANDS
ESTRANES
ESTROGENS
GLANDS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
STEROID HORMONES
STEROIDS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques