In vivo assessment of phagocytic properties of Kupffer cells
Three-compartment analysis was used to assess the kinetics of phagocytosis of Tc-99m-labeled human serum albumin microparticles (Tc-99m HSA-MM) in human Kupffer cells in vivo. The tracer turnover in these phagocytic cells could be described by a monoexponential accumulation with a two-stage elimination phase. Three-compartment analysis of the Tc-99m HSA-MM kinetics allowed us to quantify tracer attachment, phagocytosis, and degradation in Kupffer cells. The calculated time course of phagocytosis in ten control subjects proved to be identicl to that of phagocytosis of various test substances in mouse macrophage monolayers. In addition, an impairment of particle turnover at the macrophage membrane, a significantly diminished phagocytosis rate of the tracer, was observed in ten patients with various tumors.
- Research Organization:
- Nuclear Research Center, Juelich, Germany
- OSTI ID:
- 6411400
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 22:5
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
MACROPHAGES
DYNAMIC FUNCTION STUDIES
PHAGOCYTOSIS
COMPARATIVE EVALUATIONS
KINETICS
RADIOPHARMACEUTICALS
BIOLOGICAL LOCALIZATION
TECHNETIUM 99
RADIOISOTOPE SCANNING
ALBUMINS
ANIMAL CELLS
IN VIVO
ISOMERIC NUCLEI
LIVER
MAN
MICE
MICROSPHERES
PATIENTS
SCANNING ELECTRON MICROSCOPY
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DRUGS
ELECTRON MICROSCOPY
GLANDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
551001 - Physiological Systems- Tracer Techniques