Effects of acetone and fasting on cytochrome P-450 and xenobiotic metabolism in intact and hypophysectomized rats
Abstract
Hypophysectomized and intact male and female rats were fasted for 24-48 hrs or given acetone (5ml/kg body weight) in order to evaluate the effects of these treatments on hepatic microsomal cytochrome P-450 and xenobiotic metabolism. Fasting and acetone treatment resulted in a significant increase (p < 0.05) in total P-450 in intact female rats. However, there was no significant changes in P-450 in microsomes from fasted or acetone-treated hypophysectomized rats. Fasting and acetone treatment resulted in significant increases in nitrosamine metabolism in intact rats. This effect was markedly reduced in the hypophysectomized rat. When intact male rats were fasted or treated with acetone there was a significant increase in P-450 in microsomes from acetone treated rats. Aryl hydrocarbon hydroxylase activity was significantly increased in both intact and hypophysectomized male and female rats treated with acetone. These results suggest that the pituitary gland or some product markedly influences acetone-stimulated nitrosamine metabolism.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of South Florida, Tampa
- OSTI Identifier:
- 6410458
- Report Number(s):
- CONF-870644-
Journal ID: CODEN: FEPRA; TRN: 87-029117
- Resource Type:
- Conference
- Journal Name:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
- Additional Journal Information:
- Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ACETONE; BIOCHEMICAL REACTION KINETICS; MIXED-FUNCTION OXIDASES; METABOLISM; NITROSAMINES; FASTING; MICROSOMES; RATS; AMINES; ANIMALS; CELL CONSTITUENTS; ENZYMES; KETONES; KINETICS; MAMMALS; NITROSO COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANOIDS; OXIDOREDUCTASES; OXYGENASES; REACTION KINETICS; RODENTS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology
Citation Formats
Williams, M T, and Simonet, L. Effects of acetone and fasting on cytochrome P-450 and xenobiotic metabolism in intact and hypophysectomized rats. United States: N. p., 1987.
Web.
Williams, M T, & Simonet, L. Effects of acetone and fasting on cytochrome P-450 and xenobiotic metabolism in intact and hypophysectomized rats. United States.
Williams, M T, and Simonet, L. 1987.
"Effects of acetone and fasting on cytochrome P-450 and xenobiotic metabolism in intact and hypophysectomized rats". United States.
@article{osti_6410458,
title = {Effects of acetone and fasting on cytochrome P-450 and xenobiotic metabolism in intact and hypophysectomized rats},
author = {Williams, M T and Simonet, L},
abstractNote = {Hypophysectomized and intact male and female rats were fasted for 24-48 hrs or given acetone (5ml/kg body weight) in order to evaluate the effects of these treatments on hepatic microsomal cytochrome P-450 and xenobiotic metabolism. Fasting and acetone treatment resulted in a significant increase (p < 0.05) in total P-450 in intact female rats. However, there was no significant changes in P-450 in microsomes from fasted or acetone-treated hypophysectomized rats. Fasting and acetone treatment resulted in significant increases in nitrosamine metabolism in intact rats. This effect was markedly reduced in the hypophysectomized rat. When intact male rats were fasted or treated with acetone there was a significant increase in P-450 in microsomes from acetone treated rats. Aryl hydrocarbon hydroxylase activity was significantly increased in both intact and hypophysectomized male and female rats treated with acetone. These results suggest that the pituitary gland or some product markedly influences acetone-stimulated nitrosamine metabolism.},
doi = {},
url = {https://www.osti.gov/biblio/6410458},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 46:6,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1987},
month = {Fri May 01 00:00:00 EDT 1987}
}