Insulin-like growth factor I/somatomedin C: a potent inducer of oligodendrocyte development

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.3.822· OSTI ID:6410289

McMorris, F A; Smith, T M; DeSalvo, S; Furlanetto, R W

Cell cultures established from cerebrum of 1-day-old rats were used to investigate hormonal regulation of the development of oligodendrocytes, which synthesize myelin in the central nervous system. The number of oligodendrocytes that developed was preferentially increased by insulin, or by insulin-like growth factor I (IGF-I), also known as somatomedin C. High concentrations of insulin were required for substantial induction of oligodendrocyte development, whereas only 3.3 ng of IGF-I per ml was needed for a 2-fold increase in oligodendrocyte numbers. At an IGF-I concentration of 100 ng/ml, oligodendrocyte numbers were increased 6-fold in cultures grown in the presence of 10% fetal bovine serum, or up to 60-fold in cultures maintained in serum-free medium. IGF-I produced less than a 2-fold increase in the number of nonoligodendroglial cells in the same cultures. Type I IGF receptors were identified on oligodendrocytes and on a putative oligodendrocyte precursor cell population identified by using mouse monoclonal antibody A2B5. Radioligand binding assays were done. These results indicate that IGF-I is a potent inducer of oligodendrocyte development and suggest a possible mechanism based on IGF deficiency for the hypomyelination that results from early postnatal malnutrition.

• Research Organization: Wistar Institute of Anatomy and Biology, Philadelphia, PA
• OSTI ID: 6410289
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:3
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
INSULIN
BIOLOGICAL EFFECTS
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NERVE CELLS
CELL DIFFERENTIATION
STH
CELL CULTURES
CEREBRUM
CYTOCHEMISTRY
GROWTH
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CENTRAL NERVOUS SYSTEM
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HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
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ORGANS
PEPTIDE HORMONES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics