Adenovirus type 2 expresses fiber in monkey-human hybrids and reconstructed cells
Adenovirus type 2 protein expression was measured by indirect immunofluorescence in monkey-human hybrids and in cells reconstructed from monkey and human cell karyoplasts and cytoplasts. Monkey-human hybrid clones infected with adenovirus type 2 expressed fiber protein, whereas infected monkey cells alone did not. Hybrids constructed after the parental monkey cells were infected with adenovirus type 2 demonstrated that fiber synthesis in these cells could be rescued by fusion to uninfected human cells. Thus, human cells contain a dominant factor that acts in trans and overcomes the inability of monkey cells to synthesize fiber. These results are consistent with the hypothesis that the block to adenovirus replication in monkey cells involves a nuclear event that prevents the formation of functional mRNA for some late viral proteins including fiber polypeptide.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6409742
- Journal Information:
- J. Virol.; (United States), Vol. 37:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENOVIRUS
MESSENGER-RNA
ANIMAL CELLS
HYBRIDIZATION
HELA CELLS
POLYPEPTIDES
BIOSYNTHESIS
FLUORESCENCE
MONKEYS
ANIMALS
LUMINESCENCE
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PRIMATES
PROTEINS
RNA
SYNTHESIS
VERTEBRATES
VIRUSES
550700* - Microbiology
550300 - Cytology