Sulfate transport by chick renal tubule brush-border and basolateral membranes

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:6409563

Renfro, J L; Clark, N B; Metts, R E; Lynch, M A

Brush-border and basolateral membrane vesicles (BBMV and BLMV, respectively) were prepared from chick kidney by a calcium precipitation method and by centrifugation on an 8% Percoll self-generating gradient, respectively. In BBMV a 100-mM Na gluconate gradient, out>in, caused concentrative (/sup 35/S) sulfate uptake approximately fivefold greater at 1 min than at 60 min (equilibrium) whether or not the membranes were short-circuited with 100 mM K gluconate, in=out, plus 20 ..mu..g valinomycin/mg protein. A 48-mM HCO/sub 3//sup -/ gradient, in>out, stimulated a 2.5-fold higher uptake at 1 min than at 60 min, and short circuiting as above had no effect on the magnitude of this response. Imposition of a H/sup +/ gradient caused concentrative uptake fourfold higher at 1 min than at equilibrium. Short circuiting as above or addition of 0.1 mM carbonyl cyanide m-chlorophenylhydrazone (CCCP) significantly inhibited the pH gradient effect. Creation of an inside positive electrical potential with 100 mM K gluconate, out>in, plus valinomycin, also caused concentrative sulfate uptake. Based on inhibitor/competitor effects, these are distinct sulfate transport processes. In chick BLMV, imposition of an HCO/sub 3//sup -/ gradient, in>out, produced concentrative sulfate uptake. 4-Acetamido-4'-isothiocyanostilbene 2,2'-disulfonic acid disodium at 0.1 mM was an effective inhibitor of BLMV bicarbonate-sulfate exchange.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 6409563
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
RENAL CLEARANCE
SULFATES
MEMBRANE TRANSPORT
SULFUR 35
CHICKENS
DIFFUSION
INULIN
KIDNEYS
MEMBRANES
POTASSIUM COMPOUNDS
VALINOMYCIN
ALKALI METAL COMPOUNDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CARBOHYDRATES
CLEARANCE
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
EXCRETION
FOWL
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques