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Title: Identification of Schistosoma mansoni glycoproteins recognized by protective antibodies from mice immunized with irradiated cercariae

The humoral immune response of mice patently infected with Schistosoma mansoni and of mice vaccinated with radiation-attenuated cercariae were compared by radioimmunoassays and one-and two-dimensional polyacrylamide gel analyses of radioimmunoprecipitates. The binding observed with antibodies of mice vaccinated twice with radiation-attenuated cercariae over a period of 7 to 11 wk was less than 50% of the binding observed with antibodies of mice patently infected for 20 wk, but three to four times greater than that obtained with antibodies of mice infected for 6 wk, irrespective of whether the test extracts were derived from schistosomula or adult worms. Sera of vaccinated mice precipitated a restricted number of predominantly high m.w. glycoproteins of both schistosomula and adult worms metabolically labeled with sulfur-35 methionine. Each of the glycoproteins of 36 hr in vitro-cultured schistosomula that was precipitated by the sera of vaccinated mice was also precipitated by the sera of infected mice. Although radiation-attenuated larvae do not reach the adult stage, mice vaccinated with these still elicit a strong immune response against egg glycoproteins. These results show that the antibody response in mice vaccinated with radiation-attenuated larvae differs qualitatively and quantitatively from that of infected mice.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6408797
Report Number(s):
AD-A-179688/7/XAB
Resource Type:
Technical Report
Resource Relation:
Other Information: Pub. in Journal of Immunology, Vol. 136, No. 12, 4689-4694(15 Jun 1986)
Research Org:
Johns Hopkins Univ., Baltimore, MD (USA). School of Medicine
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIBODIES; IMMUNE REACTIONS; RADIOIMMUNOASSAY; MICE; RADIOPROTECTIVE SUBSTANCES; ADULTS; ANTIGENS; GLUCOPROTEINS; IMMUNITY; IRRADIATION; PLATYHELMINTHS; AGE GROUPS; ANIMALS; CARBOHYDRATES; DRUGS; HELMINTHS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC COMPOUNDS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RODENTS; SACCHARIDES; TRACER TECHNIQUES; VERTEBRATES 560130* -- Radiation Effects on Microorganisms; 560152 -- Radiation Effects on Animals-- Animals