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Title: Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies

Abstract

Radiosensitization of hypoxic bacterial cells by five 2-nitroimidazoles, with similar reduction potentials to misonidazole but having lower lipophilicites, has been measured in Escherichia coli AB 1157 and Streptococcus lactis 712. Sensitization efficiency progressively decreased with decreasing lepophilicity in E. coli but not in S. lactis. This difference is discussed in terms of the differing membrane properties of the two bacteria; E. coli resembled a multicompartment model, as would also be expected with mammalian cells. Rapid-mix experiments are described which show that the radiosensitization observed after experiments are described which show that the radiosensitization observed after preirradiation contact times between ca. 3 and 30 msec is dependent on the lipophilicity of the sensitizer, higher lipophilicity resulting in a lower contact time being required for radiosensitization. This result and the observation that a highly lipophilic compound affects only half the full oxygen enhancement level after short contact times suggest that part of the sensitization process occurs in a lipophilic compartment of the cell.

Authors:
; ;
Publication Date:
Research Org.:
Mount Vernon Hospital, Middlesex, England
OSTI Identifier:
6395191
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 85:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; RADIOSENSITIVITY; IMIDAZOLES; RADIOSENSITIVITY EFFECTS; STREPTOCOCCUS; BIOCHEMISTRY; BIOLOGICAL RADIATION EFFECTS; CALCULATION METHODS; CHEMICAL PROPERTIES; COBALT 60; CULTURE MEDIA; AZOLES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CHEMISTRY; COBALT ISOTOPES; HETEROCYCLIC COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATION EFFECTS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES; 560130* - Radiation Effects on Microorganisms; 560121 - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Anderson, R F, Patel, K B, and Sehmi, D S. Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies. United States: N. p., 1981. Web. doi:10.2307/3575420.
Anderson, R F, Patel, K B, & Sehmi, D S. Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies. United States. https://doi.org/10.2307/3575420
Anderson, R F, Patel, K B, and Sehmi, D S. 1981. "Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies". United States. https://doi.org/10.2307/3575420.
@article{osti_6395191,
title = {Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies},
author = {Anderson, R F and Patel, K B and Sehmi, D S},
abstractNote = {Radiosensitization of hypoxic bacterial cells by five 2-nitroimidazoles, with similar reduction potentials to misonidazole but having lower lipophilicites, has been measured in Escherichia coli AB 1157 and Streptococcus lactis 712. Sensitization efficiency progressively decreased with decreasing lepophilicity in E. coli but not in S. lactis. This difference is discussed in terms of the differing membrane properties of the two bacteria; E. coli resembled a multicompartment model, as would also be expected with mammalian cells. Rapid-mix experiments are described which show that the radiosensitization observed after experiments are described which show that the radiosensitization observed after preirradiation contact times between ca. 3 and 30 msec is dependent on the lipophilicity of the sensitizer, higher lipophilicity resulting in a lower contact time being required for radiosensitization. This result and the observation that a highly lipophilic compound affects only half the full oxygen enhancement level after short contact times suggest that part of the sensitization process occurs in a lipophilic compartment of the cell.},
doi = {10.2307/3575420},
url = {https://www.osti.gov/biblio/6395191}, journal = {Radiat. Res.; (United States)},
number = ,
volume = 85:3,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 1981},
month = {Sun Mar 01 00:00:00 EST 1981}
}