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Title: Sister chromatid exchange, DNA repair, and single-gene mutation

Sister chromatid exchange (SCE) has been studied in cultured mammalian cells with regard to the nature of the inducing lesion, mutation induction, and factors that modify the observed frequency following mutagen exposure, SCEs can be induced by a wide spectrum of DNA lesions and, for nine agents examined, the frequency of induced SCE is linearly related to induced single-gene mutation. Further, a deficiency in DNA repair may alter the expression of both SCE and mutation in a qualitatively similar manner. The frequency of SCE induced by mitomycin-C is suppressed in heterochromatic relative to euchromatin and, in nondividing lymphocytes, the lesions leading to the formation of SCEs may persist for several months.
Authors:
;
Publication Date:
OSTI Identifier:
6378885
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: UCLA Symp. Mol. Cell. Biol., New Ser.; (United States)
Research Org:
Lawrence Livermore National Lab., CA
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ALKYLATING AGENTS; GENETIC EFFECTS; ANIMAL CELLS; SISTER CHROMATID EXCHANGES; BENZANTHRACENE; EMS; IONIZING RADIATIONS; MITOMYCIN; NITROSO COMPOUNDS; PROFLAVINE; ULTRAVIOLET RADIATION; BIOLOGICAL REPAIR; CELL CULTURES; CHROMATIN; DNA; EXPERIMENTAL DATA; GENE MUTATIONS; MUTANTS; UREA; ACRIDINES; AMIDES; AMINES; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMITOTIC DRUGS; ANTINEOPLASTIC DRUGS; AROMATICS; AZINES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CARBONIC ACID DERIVATIVES; CHROMOSOMAL ABERRATIONS; CONDENSED AROMATICS; DATA; DRUGS; ELECTROMAGNETIC RADIATION; ESTERS; FLAVINES; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; INFORMATION; MUTAGENS; MUTATIONS; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PYRIDINES; RADIATIONS; RECOVERY; REPAIR; SULFONIC ACID ESTERS 560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987); 560121 -- Radiation Effects on Cells-- External Source-- (-1987)