Homologous radioimmunoassay for human parathyrin (residues 53-84)

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6377018

Hitzler, W; Schmidt-Gayk, H; Spiropoulos, P; Raue, F; Hufner, M

A sequential saturation double-antibody radioimmunoassay for carboxyl-terminal fragments of human parathyrin (hPTH) in serum is described. Standards are prepared with synthetic hPTH (residues 53-84) in hPTH-free serum. Antisera are obtained by immunizing guinea pigs with partly purified hPTH extracted from adenomatous glands. Tracer is prepared by labeling hPTH (53-84), presumably at the histidine residue, with /sup 125/I by the Chloramine T method at pH 8.6. Dilution curves for hPTH extracted from adenomas are superimposable on dilution curves for the synthetic 53-84 fragment. Dilution of sera from hyperparathyroid patients showed linearity of response with concentration in the present assay, but non-linearity in the heterologous radioimmunoassay. In contrast to the heterologous system, which discriminated 28 of 32 patients with primary hyperparathyroidism from 32 normals (normal range: undetectable to 54 pmol/L, omitting the highest and lowest values from controls), the present assay separated these groups without overlap.

• Research Organization: Univ. of Heidelberg, Federal Republic of Germany
• OSTI ID: 6377018
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:8
• Country of Publication: United States
• Language: English

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550601* - Medicine- Unsealed Radionuclides in Diagnostics