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Title: Polyphosphorylated fungal cell wall glycopeptides

Abstract

Penicillium charlesii secretes a 65 kDa peptidophosphogalactomannan (pPGM) containing 10 phosphodiester residues and 10 galactofuranosyl-containing galactin chains attached to a linear mannan; the polysaccharides is attached to a 3 kDa seryl- and threonyl-rich peptide. The authors have now isolated and partially characterized a form of pPGM released from mycelia of P. charlesii treated at 50/sup 0/C for 15, 30, 60 or 120 min. Two- to 3-fold more pPGM was released by heat treatment than is secreted. Crude pPGM, released by heat, was fractionated on DE-52 and was fractionated into two major fractions on the basis of its difference in negative charge. /sup 1/H-decoupled /sup 13/C NMR spectroscopy of these two fractions provided spectra very similar to that of secreted pPGM previously reported from this laboratory. /sup 1/H-decoupled /sup 31/P NMR showed major signals at 1.47, and 0.22 ppm and minor signals at 1.32, 1.15, 1.00, 0.91 and 0.76 ppm. These signals are upfield from phosphomonoesters and are in the region observed for (6-O-phosphorylcholine)- and (6-O-phosphorylethanolamine)-..cap alpha..-D-mannopyranosyl residues which are 0.22 and 0.90 ppm, respectively. These polymers contain 30 phosphodiester residues per molecule of 70 kDa mass compared with 10 phosphodiesters in secreted pPGM. Acid phosphatase and alkaline protease were themore » only lytic enzymes released by heat treatment. The evidence suggests that much of the pPGM is derived from cell walls; and that the polysaccharide is highly phosphorylated.« less

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Florida, Gainesville
OSTI Identifier:
6374525
Report Number(s):
CONF-870644-
Journal ID: CODEN: FEPRA; TRN: 87-033982
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOPROTEINS; CHEMICAL COMPOSITION; FRACTIONATION; POLYSACCHARIDES; PHOSPHORYLATION; ACID PHOSPHATASE; CARBON 13; HEAT; NMR SPECTRA; PENICILLIUM; PHOSPHORUS 31; TRACER TECHNIQUES; CARBOHYDRATES; CARBON ISOTOPES; CHEMICAL REACTIONS; ENERGY; ENZYMES; ESTERASES; EVEN-ODD NUCLEI; FUNGI; HYDROLASES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHATASES; PHOSPHORUS ISOTOPES; PLANTS; PROTEINS; SACCHARIDES; SEPARATION PROCESSES; SPECTRA; STABLE ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Bonetti, S J, Black, B, and Gander, J E. Polyphosphorylated fungal cell wall glycopeptides. United States: N. p., 1987. Web.
Bonetti, S J, Black, B, & Gander, J E. Polyphosphorylated fungal cell wall glycopeptides. United States.
Bonetti, S J, Black, B, and Gander, J E. 1987. "Polyphosphorylated fungal cell wall glycopeptides". United States.
@article{osti_6374525,
title = {Polyphosphorylated fungal cell wall glycopeptides},
author = {Bonetti, S J and Black, B and Gander, J E},
abstractNote = {Penicillium charlesii secretes a 65 kDa peptidophosphogalactomannan (pPGM) containing 10 phosphodiester residues and 10 galactofuranosyl-containing galactin chains attached to a linear mannan; the polysaccharides is attached to a 3 kDa seryl- and threonyl-rich peptide. The authors have now isolated and partially characterized a form of pPGM released from mycelia of P. charlesii treated at 50/sup 0/C for 15, 30, 60 or 120 min. Two- to 3-fold more pPGM was released by heat treatment than is secreted. Crude pPGM, released by heat, was fractionated on DE-52 and was fractionated into two major fractions on the basis of its difference in negative charge. /sup 1/H-decoupled /sup 13/C NMR spectroscopy of these two fractions provided spectra very similar to that of secreted pPGM previously reported from this laboratory. /sup 1/H-decoupled /sup 31/P NMR showed major signals at 1.47, and 0.22 ppm and minor signals at 1.32, 1.15, 1.00, 0.91 and 0.76 ppm. These signals are upfield from phosphomonoesters and are in the region observed for (6-O-phosphorylcholine)- and (6-O-phosphorylethanolamine)-..cap alpha..-D-mannopyranosyl residues which are 0.22 and 0.90 ppm, respectively. These polymers contain 30 phosphodiester residues per molecule of 70 kDa mass compared with 10 phosphodiesters in secreted pPGM. Acid phosphatase and alkaline protease were the only lytic enzymes released by heat treatment. The evidence suggests that much of the pPGM is derived from cell walls; and that the polysaccharide is highly phosphorylated.},
doi = {},
url = {https://www.osti.gov/biblio/6374525}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 46:6,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1987},
month = {Fri May 01 00:00:00 EDT 1987}
}

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