Effect of cholera toxin on cAMP levels and Na/sup +/ influx in isolated intestinal epithelial cells
Freshly isolated chicken intestinal cells contain approximately 20 pmol adenosine 3',5'-cyclic monophosphate (cAMP)/mg cellular protein. Incubation with 3 ..mu..g/ml cholera toxin (CT) at 37/sup 0/C induces an elevation of cellular cAMP beginning 10-15 min after initial exposure. The response is linear with time for 40-50 min and causes a six- to eightfold increase over control levels at steady state. Dibutyryl cAMP and agents that increase cAMP production inhibit Na/sup +/ influx into the isolated enterocytes. Chlorpromazine completely abolishes the toxin-induced elevation of cAMP in the isolated cells and also reverses the effect on Na/sup +/ entry. The data provide evidence for a cAMP-mediated control of intestinal cell Na/sup +/ uptake, which may represent the mechanistic basis for the antiabsorptive effect of CT on Na/sup +/ during induction of intestinal secretory activity. Studies on the time-dependent effects of chlorpromazine on both intracellular cAMP concentration and Na/sup +/ influx suggest that the reactivation of the Na/sup +/ transport system after cAMP-induced inhibition is slow relative to the disappearance of cAMP.
- Research Organization:
- Univ. of Rochester Medical Center, NY
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6373507
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 243:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMP
BIOCHEMICAL REACTION KINETICS
CATIONS
MEMBRANE TRANSPORT
TOXINS
BIOLOGICAL EFFECTS
CHICKENS
CHLORPROMAZINE
CHOLERA
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
SODIUM 22
SODIUM COMPOUNDS
SOMATIC CELLS
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
AZINES
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIRDS
BODY
CENTRAL NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHARGED PARTICLES
DISEASES
DRUGS
FOWL
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
INFECTIOUS DISEASES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTHIAZINES
PSYCHOTROPIC DRUGS
RADIOISOTOPES
REACTION KINETICS
SODIUM ISOTOPES
TISSUES
TOXIC MATERIALS
TRANQUILIZERS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques