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Title: Cloning and sequencing of human intestinal alkaline phosphatase cDNA

Abstract

Partial protein sequence data obtained on intestinal alkaline phosphatase indicated a high degree of homology with the reported sequence of the placental isoenzyme. Accordingly, placental alkaline phosphatase cDNA was cloned and used as a probe to clone intestinal alkaline phosphatase cDNA. The latter is somewhat larger (3.1 kilobases) than the cDNA for the placental isozyme (2.8 kilobases). Although the 3' untranslated regions are quite different, there is almost 90% homology in the translated regions of the two isozymes. There are, however, significant differences at their amino and carboxyl termini and a substitution of an alanine in intestinal alkaline phosphatase for a glycine in the active site of the placental isozyme.

Authors:
; ; ;
Publication Date:
Research Org.:
Roche Institute of Molecular Biology, Nutley, NJ
OSTI Identifier:
6372767
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; RECOMBINANT DNA; DNA SEQUENCING; DNA-CLONING; ATP; INTESTINES; ISOENZYMES; MAN; MOLECULAR STRUCTURE; PHOSPHORUS 32; PLACENTA; SULFUR 35; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CLONING; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; ENZYMES; ESTERASES; EVEN-ODD NUCLEI; FETAL MEMBRANES; GASTROINTESTINAL TRACT; HYDROLASES; ISOTOPES; LIGHT NUCLEI; MAMMALS; MEMBRANES; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHATASES; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; SULFUR ISOTOPES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Berger, J, Garattini, E, Hua, J C, and Udenfriend, S. Cloning and sequencing of human intestinal alkaline phosphatase cDNA. United States: N. p., 1987. Web. doi:10.1073/pnas.84.3.695.
Berger, J, Garattini, E, Hua, J C, & Udenfriend, S. Cloning and sequencing of human intestinal alkaline phosphatase cDNA. United States. https://doi.org/10.1073/pnas.84.3.695
Berger, J, Garattini, E, Hua, J C, and Udenfriend, S. 1987. "Cloning and sequencing of human intestinal alkaline phosphatase cDNA". United States. https://doi.org/10.1073/pnas.84.3.695.
@article{osti_6372767,
title = {Cloning and sequencing of human intestinal alkaline phosphatase cDNA},
author = {Berger, J and Garattini, E and Hua, J C and Udenfriend, S},
abstractNote = {Partial protein sequence data obtained on intestinal alkaline phosphatase indicated a high degree of homology with the reported sequence of the placental isoenzyme. Accordingly, placental alkaline phosphatase cDNA was cloned and used as a probe to clone intestinal alkaline phosphatase cDNA. The latter is somewhat larger (3.1 kilobases) than the cDNA for the placental isozyme (2.8 kilobases). Although the 3' untranslated regions are quite different, there is almost 90% homology in the translated regions of the two isozymes. There are, however, significant differences at their amino and carboxyl termini and a substitution of an alanine in intestinal alkaline phosphatase for a glycine in the active site of the placental isozyme.},
doi = {10.1073/pnas.84.3.695},
url = {https://www.osti.gov/biblio/6372767}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:3,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 1987},
month = {Sun Feb 01 00:00:00 EST 1987}
}