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Title: Purification and characterization of lead-induced zinc thionein in the liver of rats

Metallothioneins are involved in homeostasis of essential metals, but its exact biological function remains obscure. Recently it was observed that the administration of lead increased zinc content in the low molecular-weight protein fraction in the rat hepatic 105000x g soluble fraction by using Sephadex G-75 gel filtration, and that this fraction contained only zinc, not lead, as metal. Therefore, the authors have investigated the effects of some drugs on this zinc contained low molecular-weight protein (zinc binding protein, Zn-BP), and further isolated and purified the ZN-BP from liver of rats treated with lead.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6371683
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bull. Environ. Contam. Toxicol.; (United States); Journal Volume: 35:2
Research Org:
Nagasaki Univ., Japan
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEAD COMPOUNDS; BIOLOGICAL EFFECTS; METALLOTHIONEIN; BIOLOGICAL FUNCTIONS; CHEMICAL PREPARATION; ABSORPTION SPECTROSCOPY; ACETATES; GEL PERMEATION CHROMATOGRAPHY; LEAD CHLORIDES; LIVER; RATS; ZINC; ANIMALS; BODY; CARBOXYLIC ACID SALTS; CHLORIDES; CHLORINE COMPOUNDS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; ELEMENTS; FUNCTIONS; GLANDS; HALIDES; HALOGEN COMPOUNDS; LEAD HALIDES; MAMMALS; METALLOPROTEINS; METALS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SEPARATION PROCESSES; SPECTROSCOPY; SYNTHESIS; VERTEBRATES 560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)