Prevention of graft rejection in allogeneic bone marrow transplantation. II. Preclinical studies with three radiation protocols

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Transplantation; (USA)

DOI:https://doi.org/10.1097/00007890-199009000-00010· OSTI ID:6366870

Malilay, G P; Sevenich, E A; Filipovich, A H ^[1]

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1. Univ. of Minnesota, Minneapolis (USA)

Three radiotherapeutic regimens were compared in vitro to determine their immunosuppressive potential against non-MHC-restricted cytotoxic cells. Assays of natural killer and lymphokine-activated killer function, and cytotoxicity against allogeneic cells were used to quantitate the cytotoxic potential of peripheral blood mononuclear cells from healthy individuals following irradiation with a single dose of 1000 cGy on day 0, 1320 cGy of fractionated radiation (165 cGy b.i.d. x 4 days), or split-dose irradiation consisting of 1000 cGy on day 0 followed 5 or 7 days later by 500 cGy. Both irradiated and nonirradiated (control) PBMC cultures were maintained in culture with medium containing interleukin-2, immunophenotyped, and assayed for cytotoxicity from 1 to 8 days after irradiation. Single dose and fractionated-dose irradiation resulted in a progressive decline in cytotoxic capacity, with an 80% inhibition of both NK and LAK cell activity 8 days after onset of irradiation. The split dose of 500 cGy administered 7 days after a dose of 1000 cGy was found to be the most effective in eliminating NK (93% inhibition) and LAK (100% inhibition) cytotoxicity. These data indicate that split-dose irradiation may result in greater immunosuppression than single-dose or fractionated irradiation.

• OSTI ID: 6366870
• Journal Information: Transplantation; (USA), Vol. 50:3; ISSN 0041-1337
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
TRANSPLANTS
IMMUNITY
RADIOINDUCTION
FRACTIONATED IRRADIATION
GRAFT-HOST REACTION
IMMUNOSUPPRESSION
LYMPHOCYTES
LYMPHOKINES
MONOCYTES
RADIOTHERAPY
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
IRRADIATION
LEUKOCYTES
MATERIALS
MEDICINE
MITOGENS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
PROTEINS
RADIOLOGY
SOMATIC CELLS
THERAPY
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture