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Title: Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase

Abstract

To elucidate the gene source of the UDP-glucuronyltransferase (UDP-GT) family of enzymes, the authors have investigated the effects on the induction of p-nitrophenol UDP-GT by a combined treatment of rats with the carcinogen, 3-methylcholanthrene (3-MC) and the phenolic antioxidant, butylated hydroxyanisole (BHA). In this study, the elution profile of the enzyme was determined by ion exchange and affinity chromatography. P-nitrophenol UDP-GT activity was found to elute in the salt fractions of a 0-500 mM KCl gradient and required at least 5 mM UDP-glucuronic acid (UDPGA) to elute from the affinity column and has a M/sub r/ of 56,000. The results indicate that BHA + 3-MC is a more potent inducer of UDP-GT compared to either of the inducers used alone. For further investigation of the molecular basis of the enhanced induction, a cDNA library was generated from the BHA + 3-MC treated rat liver mRNA, and some mRNA from the same treatment was in vitro translated to establish the identities of the products compared to the in vivo translated proteins from the different treatments. The authors results suggest that the BHA and 3-MC induce the expression of different UDP-GT genes and the increased induction is due to these respective genes.

Authors:
; ; ;
Publication Date:
Research Org.:
Atlanta Univ., GA
OSTI Identifier:
6366483
Report Number(s):
CONF-870644-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 3-METHYLCHOLANTHRENE; BIOLOGICAL EFFECTS; ANTIOXIDANTS; TRANSFERASES; ENZYME ACTIVITY; CARCINOGENS; GENES; ION EXCHANGE CHROMATOGRAPHY; LIVER; MESSENGER-RNA; RATS; ANIMALS; AROMATICS; BODY; CHROMATOGRAPHY; DIGESTIVE SYSTEM; ENZYMES; GLANDS; HYDROCARBONS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; POLYCYCLIC AROMATIC HYDROCARBONS; RNA; RODENTS; SEPARATION PROCESSES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Ananaba, G A, Stewart, J, Boston, C, and McCrary, J. Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase. United States: N. p., 1987. Web.
Ananaba, G A, Stewart, J, Boston, C, & McCrary, J. Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase. United States.
Ananaba, G A, Stewart, J, Boston, C, and McCrary, J. 1987. "Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase". United States.
@article{osti_6366483,
title = {Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase},
author = {Ananaba, G A and Stewart, J and Boston, C and McCrary, J},
abstractNote = {To elucidate the gene source of the UDP-glucuronyltransferase (UDP-GT) family of enzymes, the authors have investigated the effects on the induction of p-nitrophenol UDP-GT by a combined treatment of rats with the carcinogen, 3-methylcholanthrene (3-MC) and the phenolic antioxidant, butylated hydroxyanisole (BHA). In this study, the elution profile of the enzyme was determined by ion exchange and affinity chromatography. P-nitrophenol UDP-GT activity was found to elute in the salt fractions of a 0-500 mM KCl gradient and required at least 5 mM UDP-glucuronic acid (UDPGA) to elute from the affinity column and has a M/sub r/ of 56,000. The results indicate that BHA + 3-MC is a more potent inducer of UDP-GT compared to either of the inducers used alone. For further investigation of the molecular basis of the enhanced induction, a cDNA library was generated from the BHA + 3-MC treated rat liver mRNA, and some mRNA from the same treatment was in vitro translated to establish the identities of the products compared to the in vivo translated proteins from the different treatments. The authors results suggest that the BHA and 3-MC induce the expression of different UDP-GT genes and the increased induction is due to these respective genes.},
doi = {},
url = {https://www.osti.gov/biblio/6366483}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 46:6,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1987},
month = {Fri May 01 00:00:00 EDT 1987}
}

Conference:
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