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Title: Lasting engraftment of histoincompatible bone marrow cells in dogs

Conditioning protocols were tested for their efficacy in increasng the incidence of engraftment of histoincompatible dog bone marrow cells. Cyclophosphamide and total body irradiation (TBI), Corynebacterium parvum and TBI, a 3- or 5-day delayed transfusion of bone marrow cells after TBI, or an increase in the number of donor bone marrow cells or lymphocytes appeared to be ineffective. These protocols were previously reported to promote recovery of splenic hemopoiesis in mice in short-term assays. The noted discrepancy between studies with mice and dogs invalidated allogeneic resistance as measured in the mouse spleen assay as a model for bone marrow allograft rejection. Intravenous treatment with silica particles or L-asparaginase did improve the engraftment rate after 7.5 Gy TBI. Low efficiency and significant extra toxicity restrict the applicability of these procedures. The most promising conditioning schedule found appeared to be two fractions of 6.0 Gy TBI separated by a 72-h interval. Prolonged survival was noted after transplantation of bone marrow cells from a one-DLA haplotype-mismatched donor. Possibilities for further improvement of this protocol are discussed.
Authors:
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Publication Date:
OSTI Identifier:
6355364
Resource Type:
Journal Article
Resource Relation:
Journal Name: Transplantation; (United States); Journal Volume: 31:5
Research Org:
Radiobiological Inst. TNO, Rijswijk, Netherlands
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; BONE MARROW CELLS; TRANSPLANTS; BIOLOGICAL RADIATION EFFECTS; DOGS; DRUGS; GRAFT-HOST REACTION; LEUKEMIA; SPLEEN; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BODY; CONNECTIVE TISSUE CELLS; DISEASES; EXTERNAL IRRADIATION; HEMIC DISEASES; IRRADIATION; MAMMALS; NEOPLASMS; ORGANS; RADIATION EFFECTS; SOMATIC CELLS; VERTEBRATES 560121* -- Radiation Effects on Cells-- External Source-- (-1987); 550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-); 550200 -- Biochemistry