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Title: Regional and laminar distribution of the dopamine and serotonin innervation in the macaque cerebral cortex: a radioautographic study

Abstract

The regional density and laminar distribution of dopamine (DA) and serotonin (5-HT) afferents were investigated in the cerebral cortex of cynomolgus monkeys using a radioautographic technique that is based on the high affinity uptake capacity of these aminergic neurons. Large vibratome sections, 50 micron thick, were incubated with (3H) DA (0.2 microM) and desipramine (5 microM) or with unlabeled norepinephrine (5 microM) and (3H) 5-HT (0.6 microM), which allowed for the specific labeling of the DA and 5-HT innervations, respectively. After fixation, these sections were dried, defatted, and radioautographed by dipping. Semiquantitative data on the DA innervation also were provided by counting (3H) DA-labeled axonal varicosities in radioautographs from 4-micron-thick sections of the slices obtained after epon embedding. The DA innervation was widespread and differed in density and laminar distribution in the agranular and granular cortices. DA afferents were densest in the anterior cingulate (area 24) and the motor areas (areas 4, 6, and supplementary motor area (SMA)). In the latter they displayed a trilaminar pattern of distribution, predominating in layers I, IIIa, and V-VI, with characteristic cluster-like formations in layer IIIa, especially in the medial part of motor areas. In the granular prefrontal (areas 46, 9, 10, 11, 12),more » parietal (areas 1, 2, 3, 5, 7), temporal (areas 21, 22), and posterior cingulate (area 23) cortices, DA afferents were less dense and showed a bilaminar pattern of distribution, predominating in the depth of layer I and in layers V-VI; density in layers II, III, and IV was only 20% of that in layer I. The lowest density was in the visual cortex, particularly in area 17, where the DA afferents were almost restricted to layer I.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Hopital Salpetriere, Paris (France)
OSTI Identifier:
6354152
Resource Type:
Journal Article
Journal Name:
J. Comp. Neurol.; (United States)
Additional Journal Information:
Journal Volume: 273:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DOPAMINE; SUBCELLULAR DISTRIBUTION; SEROTONIN; AUTORADIOGRAPHY; CEREBRAL CORTEX; NERVES; NEUROLOGY; RATS; TRITIUM COMPOUNDS; AMINES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BODY; BRAIN; CARDIOTONICS; CARDIOVASCULAR AGENTS; CENTRAL NERVOUS SYSTEM; CEREBRUM; DISTRIBUTION; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; LABELLED COMPOUNDS; MAMMALS; MEDICINE; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; PYRROLES; RADIOPROTECTIVE SUBSTANCES; RODENTS; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Berger, B, Trottier, S, Verney, C, Gaspar, P, and Alvarez, C. Regional and laminar distribution of the dopamine and serotonin innervation in the macaque cerebral cortex: a radioautographic study. United States: N. p., 1988. Web. doi:10.1002/cne.902730109.
Berger, B, Trottier, S, Verney, C, Gaspar, P, & Alvarez, C. Regional and laminar distribution of the dopamine and serotonin innervation in the macaque cerebral cortex: a radioautographic study. United States. https://doi.org/10.1002/cne.902730109
Berger, B, Trottier, S, Verney, C, Gaspar, P, and Alvarez, C. 1988. "Regional and laminar distribution of the dopamine and serotonin innervation in the macaque cerebral cortex: a radioautographic study". United States. https://doi.org/10.1002/cne.902730109.
@article{osti_6354152,
title = {Regional and laminar distribution of the dopamine and serotonin innervation in the macaque cerebral cortex: a radioautographic study},
author = {Berger, B and Trottier, S and Verney, C and Gaspar, P and Alvarez, C},
abstractNote = {The regional density and laminar distribution of dopamine (DA) and serotonin (5-HT) afferents were investigated in the cerebral cortex of cynomolgus monkeys using a radioautographic technique that is based on the high affinity uptake capacity of these aminergic neurons. Large vibratome sections, 50 micron thick, were incubated with (3H) DA (0.2 microM) and desipramine (5 microM) or with unlabeled norepinephrine (5 microM) and (3H) 5-HT (0.6 microM), which allowed for the specific labeling of the DA and 5-HT innervations, respectively. After fixation, these sections were dried, defatted, and radioautographed by dipping. Semiquantitative data on the DA innervation also were provided by counting (3H) DA-labeled axonal varicosities in radioautographs from 4-micron-thick sections of the slices obtained after epon embedding. The DA innervation was widespread and differed in density and laminar distribution in the agranular and granular cortices. DA afferents were densest in the anterior cingulate (area 24) and the motor areas (areas 4, 6, and supplementary motor area (SMA)). In the latter they displayed a trilaminar pattern of distribution, predominating in layers I, IIIa, and V-VI, with characteristic cluster-like formations in layer IIIa, especially in the medial part of motor areas. In the granular prefrontal (areas 46, 9, 10, 11, 12), parietal (areas 1, 2, 3, 5, 7), temporal (areas 21, 22), and posterior cingulate (area 23) cortices, DA afferents were less dense and showed a bilaminar pattern of distribution, predominating in the depth of layer I and in layers V-VI; density in layers II, III, and IV was only 20% of that in layer I. The lowest density was in the visual cortex, particularly in area 17, where the DA afferents were almost restricted to layer I.},
doi = {10.1002/cne.902730109},
url = {https://www.osti.gov/biblio/6354152}, journal = {J. Comp. Neurol.; (United States)},
number = ,
volume = 273:1,
place = {United States},
year = {Fri Jul 01 00:00:00 EDT 1988},
month = {Fri Jul 01 00:00:00 EDT 1988}
}