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Title: Decreased ATPase activity in adriamycin nephrosis is independent of proteinuria

Abstract

In previous studies from this laboratory it has been shown that ATP-ase activity in situ in the glomerular basement membrane (GBM) is clearly reduced in rats rendered nephrotic after treatment with adriamycin (ADR). The question was raised whether this reduction of ATP-ase activity in the GBM is due to toxic activity of ADR or rather a result of the nephrotic condition per se. Therefore, we studied ATP-ase activity using the cerium-based method in kidneys from ADR-treated rats without proteinuria (48 hr after ADR injection), or with proteinuria (approximately 150 mg/24 hr) several weeks after ADR injection. Also kidneys from rats rendered nephrotic by surgical ablation and from non-nephrotic rats treated with local X-irradiation (2000 rads) as well as from normal control rats were studied. The results show that in the GBM of ADR-treated or irradiated rats, clear reduction of ATP-ase activity is observed irrespective of their proteinuria, whereas in the GBM of rats rendered nephrotic by renal ablation (approximately 156 mg/24 hr mean protein excretion) no reduction of enzyme activity is found. It is concluded that decreased ATP-ase activity of the glomerular filtration barrier in ADR-treated rats is due to an early toxic activity of this drug and not amore » result of the nephrotic state per se. In view of the identical results in X-irradiated rats, it is likely that ADR may act through production of toxic radicals leading to damage of this membrane-associated enzyme system.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Groningen, The Netherlands
OSTI Identifier:
6351926
Resource Type:
Journal Article
Journal Name:
Kidney Int.; (United States)
Additional Journal Information:
Journal Volume: 3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ATP-ASE; ENZYME ACTIVITY; DOXORUBICIN; TOXICITY; KIDNEYS; BIOLOGICAL RADIATION EFFECTS; UROGENITAL SYSTEM DISEASES; PATHOGENESIS; ENDOTHELIUM; EPITHELIUM; NEPHRECTOMY; RATS; X RADIATION; ACID ANHYDRASES; ANIMAL TISSUES; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTINEOPLASTIC DRUGS; BIOLOGICAL EFFECTS; BODY; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; HYDROLASES; IONIZING RADIATIONS; MAMMALS; MEDICINE; ORGANS; PHOSPHOHYDROLASES; RADIATION EFFECTS; RADIATIONS; RODENTS; SURGERY; TISSUES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Bakker, W W, Kalicharan, D, Donga, J, Hulstaert, C E, and Hardonk, M J. Decreased ATPase activity in adriamycin nephrosis is independent of proteinuria. United States: N. p., 1987. Web. doi:10.1038/ki.1987.55.
Bakker, W W, Kalicharan, D, Donga, J, Hulstaert, C E, & Hardonk, M J. Decreased ATPase activity in adriamycin nephrosis is independent of proteinuria. United States. https://doi.org/10.1038/ki.1987.55
Bakker, W W, Kalicharan, D, Donga, J, Hulstaert, C E, and Hardonk, M J. 1987. "Decreased ATPase activity in adriamycin nephrosis is independent of proteinuria". United States. https://doi.org/10.1038/ki.1987.55.
@article{osti_6351926,
title = {Decreased ATPase activity in adriamycin nephrosis is independent of proteinuria},
author = {Bakker, W W and Kalicharan, D and Donga, J and Hulstaert, C E and Hardonk, M J},
abstractNote = {In previous studies from this laboratory it has been shown that ATP-ase activity in situ in the glomerular basement membrane (GBM) is clearly reduced in rats rendered nephrotic after treatment with adriamycin (ADR). The question was raised whether this reduction of ATP-ase activity in the GBM is due to toxic activity of ADR or rather a result of the nephrotic condition per se. Therefore, we studied ATP-ase activity using the cerium-based method in kidneys from ADR-treated rats without proteinuria (48 hr after ADR injection), or with proteinuria (approximately 150 mg/24 hr) several weeks after ADR injection. Also kidneys from rats rendered nephrotic by surgical ablation and from non-nephrotic rats treated with local X-irradiation (2000 rads) as well as from normal control rats were studied. The results show that in the GBM of ADR-treated or irradiated rats, clear reduction of ATP-ase activity is observed irrespective of their proteinuria, whereas in the GBM of rats rendered nephrotic by renal ablation (approximately 156 mg/24 hr mean protein excretion) no reduction of enzyme activity is found. It is concluded that decreased ATP-ase activity of the glomerular filtration barrier in ADR-treated rats is due to an early toxic activity of this drug and not a result of the nephrotic state per se. In view of the identical results in X-irradiated rats, it is likely that ADR may act through production of toxic radicals leading to damage of this membrane-associated enzyme system.},
doi = {10.1038/ki.1987.55},
url = {https://www.osti.gov/biblio/6351926}, journal = {Kidney Int.; (United States)},
number = ,
volume = 3,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 1987},
month = {Sun Mar 01 00:00:00 EST 1987}
}