Effects of exposing rat embryos in utero to physical or chemical teratogens are expressed later as enhanced induction of heat-shock proteins when embryonic hearts are cultured in vitro

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Teratog., Carcinog., Mutagen.; (United States)

DOI:https://doi.org/10.1002/tcm.1770080602· OSTI ID:6350526

Higo, H; Higo, K; Lee, J Y; Hori, H; Satow, Y

In order to get more insight into the effects of teratogens on developing embryos, we investigated the protein synthesis patterns of the target organs isolated from teratogen-treated embryos. Rat embryos were either irradiated in utero with either 252Cf fission neutrons or 60Co gamma rays on day 8 of gestation or treated in utero with a bis(dichloroacetyl)diamine (a chemical teratogen) on days 9 and 10. Hearts were removed from the embryos on day 12 and were incubated in vitro at 37 degrees C in the presence of (35S)methionine for up to 8 hr. The newly synthesized labeled proteins were then analyzed qualitatively by two-dimensional polyacrylamide gel electrophoresis. Enhanced and prolonged induction of a family of heat-shock (stress) proteins with a molecular weight of about 70,000 (SP70s) was observed as compared with those of controls. Among the teratogen-treated hearts, those with gross malformations already detectable at this early stage showed especially higher inductions of SP70s than did the others. The abnormal expression of SP70s observed in the present study appears to be a reflection of persisting cellular (tissue) damage inflicted by the teratogens, and the extent of the induction may be indicative of the degree and/or type of the damage. Such persisting defects in surviving cells, manifested by abnormal induction of SP70s in the present study, might be related to malformation of embryonic hearts.

• Research Organization: Hiroshima Univ. (Japan)
• OSTI ID: 6350526
• Journal Information: Teratog., Carcinog., Mutagen.; (United States), Vol. 8:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
HEART
CONGENITAL MALFORMATIONS
PROTEINS
BIOSYNTHESIS
TERATOGENS
TOXICITY
CALIFORNIUM 252
COBALT 60
EMBRYOS
GAMMA RADIATION
GENE REGULATION
METHIONINE
MOLECULAR WEIGHT
MYOCARDIUM
PREGNANCY
RATS
SULFUR 35
TERATOGENESIS
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALIFORNIUM ISOTOPES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
HEAVY NUCLEI
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MALFORMATIONS
MAMMALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
RADIATIONS
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals
560300 - Chemicals Metabolism & Toxicology
550901 - Pathology- Tracer Techniques