Enzymatic hydrolysis of cellulose in aqueous two-phase systems. 1. Partition of cellulases from Trichoderma reesei
The partitioning of endo-..beta..-glucanase, exo-..beta..-glucananse, and ..beta..-glucosidase from Trichoderma reesei QM 9414 in aqueous two-phase systems has been studied with the object of designing a phase system for continuous bioconversion of cellulose. The partitioning of the enzymes in two-phase systems composed of various water soluble polymeric compounds were studied. Systems based on dextran and polyethylene glycol (PEG) were optimal for one sidedly partitioning of the enzymes to the bottom phase. The influence of polymer molecular weights, polymer concentration, ionic composition of the medium, pH, temperature, and adsorption of the enzymes to cellulose on the enyzme partition coefficients (K) were studied. By combining the effects of polymer molecular weight and adsorption to cellulose, K values could be reduced for endo-..beta..-glucanase to 0.02 and for ..beta..-glucosidase to 0.005 at 20 degrees C in a phase system of Dvalues could be reduced for endo-..beta..-glucanase to 0.02 and for ..beta..-glucosidase to 0.005 at 20 degrees C in a phase system of Dextran 40-PEG 40000 in the presence of excess cellulose. At 50 degrees C, K values were increased by a factor of two. In a phase system based on inexpensive crude dextran and PEG, the partition coefficient for endo-..beta..-glucanase was 0.16 and for beta-glucosidase was 0.14 at 20 degrees C with excess cellulose present.
- Research Organization:
- Lund Univ. (Sweden)
- OSTI ID:
- 6335463
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 27:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
FRACTIONATION
CELLULOSE
ENZYMATIC HYDROLYSIS
AQUEOUS SOLUTIONS
BIOCONVERSION
CONTINUOUS CULTURE
DILUTION
ENZYMES
GLUCOSIDASE
SUBSTRATES
TRICHODERMA VIRIDE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISPERSIONS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MIXTURES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SOLUTIONS
SOLVOLYSIS
TRICHODERMA
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