Post-mortem storage of tissue for X-ray microanalysis in pathology
Possible alternatives to rapid freezing in liquid nitrogen of tissue for X-ray microanalysis of electrolytes at the cellular level were investigated. These alternatives might be used in cases where tissue becomes available for examination, e.g., at autopsy, but liquid nitrogen is not immediately available. Rat submandibular gland was used as a test tissue. Freezing of pieces of tissue in a conventional freezer at -80 degrees C or even at -20 degrees C retained the elemental distribution at the cellular level, and also retained the difference between a normal and a pathological situation. Storage of tissue in a refrigerator, or delaying the autopsy in anticipation of the arrival of liquid nitrogen is not recommended. Significant changes in the cellular ion content occurred if the tissue was left in the animal for 24h post-mortem.
- Research Organization:
- Wenner-Gren Institute, Stockholm, Sweden
- OSTI ID:
- 6334027
- Journal Information:
- Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CYTOLOGICAL TECHNIQUES
EVALUATION
SALIVARY GLANDS
ELECTRON MICROPROBE ANALYSIS
AUTOPSY
FREEZING
METALS
PATHOLOGY
RATS
TEMPERATURE EFFECTS
ANIMALS
BODY
CHEMICAL ANALYSIS
DIAGNOSTIC TECHNIQUES
ELEMENTS
GLANDS
MAMMALS
MICROANALYSIS
ORGANS
RODENTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics