(Controls of the plant endomembrane-secretory pathway): Performance report
This project has been directed towards an understanding of the cellular and molecular mechanisms by which higher plants control the composition of the plasma membrane, through analysis of the biosynthesis, modification and targeting of plasma membrane proteins and glycoproteins. We have undertaken an identification of molecular markers both for the plasma membrane and for the biosynthetic processes, and the development of techniques for the isolation of conditional-lethal mutants defective at defined stages within the endomembrane-secretory pathway responsible for the biosynthesis, modification and targeting of plasma membrane proteins and glycoproteins. For the identification of molecular markers for the plasma membrane, monoclonal antibodies directed against epitopes present at the plant cell surface have been developed. Novel molecular markers for the plant plasma membrane and for the endomembrane-secretory pathway have been sought. Methods for the analysis of beta-glucuronidase in higher plants have been developed. These technologies have involved the use of flow cytometry and fluorescence-activated cell sorting. In addition, we have been investigating the feasibility of expression of animal plasma membrane marker proteins in plants, specifically the VSV G-protein. 5 refs., 6 figs.
- Research Organization:
- Nebraska Univ., Lincoln (USA)
- DOE Contract Number:
- FG02-85ER13352
- OSTI ID:
- 6327256
- Report Number(s):
- DOE/ER/13352-3; ON: DE87013295
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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GLUCOPROTEINS
MOLECULAR BIOLOGY
TWO-DIMENSIONAL ELECTROPHORESIS
GLUCURONIDASE
DNA-CLONING
GENE REGULATION
HYBRIDOMAS
DESIGN
PLANT CELLS
CELL FLOW SYSTEMS
PROTEINS
CELL MEMBRANES
DNA SEQUENCING
GENE MUTATIONS
MONOCLONAL ANTIBODIES
NICOTIANA
PLASMIDS
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ANIMAL CELLS
ANTIBODIES
CARBOHYDRATES
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DISTRIBUTION
ELECTROPHORESIS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MEMBRANES
MUTATIONS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
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