High-performance liquid chromatographic determination of azaarenes and their metabolites in groundwater affected by creosote wood preservatives
- Univ. of Wisconsin-Stout, Menomonie (USA)
Polynuclear azaheterocyclic compounds (azaarenes) are nitrogen-containing analogs of polycyclic aromatic hydrocarbons (PAHs). The nitrogen atom in the ring system causes these compounds to be slightly polar and considerably more water soluble than related PAHs. A method using a solid-surface sorption technique to extract and concentrate azaarenes and their principle metabolites present in groundwater that contains creosote waste is described. Analyte isolation and concentration is accomplished by solid-phase extraction on n-octadecyl cartridges followed by instrumental determination involving high-performance liquid chromatography. Separations and detection are achieved using flexible-walled, wide-bore columns with ultraviolet and fluorescence photometric detectors connected in series. Fluorescence detection alone is insufficient because the fluorescence response produced by two-ring azaarenes is limited. Short wavelength (229 nm) absorbance detection provides improved sensitivity for these compounds and peak rationing for more definitive identification. In this study, oxygen-containing metabolites of quinoline, isoquinoline, and acridine are detected in groundwater from hazardous waste sites in Pensacola, Florida and St. Louis Park, Minnesota. Concentrations ranging from mg/L to ng/L are measured. The dependence of measured octanol-water partition coefficients on pH is discussed in the context of the isolation chemistry. As a direct bacterial degradation product of acridine with a relatively long environmental persistence, 9-acridinone may serve as a biogenic marker signaling creosote contamination of groundwater.
- OSTI ID:
- 6326675
- Journal Information:
- Journal of Chromatographic Science; (USA), Vol. 28:6; ISSN 0021-9665
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AZAARENES
LIQUID COLUMN CHROMATOGRAPHY
GROUND WATER
CONTAMINATION
METABOLITES
WATER POLLUTION
MINNESOTA
CREOSOTE
ECOLOGICAL CONCENTRATION
FLUORESCENCE
AROMATICS
CHROMATOGRAPHY
FEDERAL REGION V
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LUMINESCENCE
NORTH AMERICA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
SEPARATION PROCESSES
USA
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)