Hepatic monooxygenase induction and promutagen activation in channel catfish from a contaminated river basin

Journal Article · Thu Dec 01 00:00:00 EST 1988 · Ecotoxicol. Environ. Saf.; (United States)

DOI:https://doi.org/10.1016/0147-6513(88)90055-3· OSTI ID:6323513

Winston, G W; Shane, B S; Henry, C B

To better understand the etiology of cancer in fish from polluted waters, the impact of environmental contaminants on xenobiotic metabolism of channel catfish (Ictalurus punctatus) from a highly polluted water body, Devil's Swamp in southeastern Louisiana, has been investigated. Fish from Devil's Swamp bioaccumulated polynuclear aromatic hydrocarbons (PAH), chlorinated hydrocarbon insecticides (CHI), and polychlorinated biphenyls (PCB) in fat tissue, the latter exceeding 7000 ppb. Reference catfish from the University farm, Ben Hur, were virtually devoid of PAH, CHI, and PCB. Liver microsomal enzymes (MFO) from Devil's Swamp fish were markedly induced. The specific content of cytochromes P450 and b5 and the specific activities of NAD(P)H-cytochrome c reductase were two to three times higher than those of Ben Hur fish. Consistent with this induction, a 9000g supernatant from Devil's Swamp but not Ben Hur fish activated 2-aminofluorene and benzo(a)pyrene (BP) to mutagens in the Ames test. BP metabolism by Devil's Swamp fish liver microsomes was inhibited to a greater extent by alpha-naphthoflavone than was BP metabolism by Ben Hur fish microsomes. This finding indicates that the induced activity in the Devil's Swamp fish liver was the result of P450 isozymes characteristic of PAH/PCB induction. Thus, exposure of fish to environmental pollutants can alter MFO leading to enhanced metabolic activation of promutagens to mutagens.

• Research Organization: Louisiana State Univ., Baton Rouge (USA)
• OSTI ID: 6323513
• Journal Information: Ecotoxicol. Environ. Saf.; (United States), Vol. 16:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZOPYRENE
CARCINOGEN SCREENING
CHLORINATED AROMATIC HYDROCARBONS
BIOLOGICAL ACCUMULATION
FISHES
NEOPLASMS
FLUORENE
ETIOLOGY
OXYGENASES
ENZYME INDUCTION
POLYCYCLIC AROMATIC HYDROCARBONS
CYTOCHROMES
LIVER
METABOLIC ACTIVATION
MICROSOMES
MUTAGENS
TOXICITY
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
CELL CONSTITUENTS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DISEASES
ENZYMES
GENE REGULATION
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
POLLUTION
PROTEINS
SCREENING
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology