Analysis of binding of a technetium-99m-labeled monoclonal antibody to lentivirus-infected cells

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Investigative Radiology; (USA)

DOI:https://doi.org/10.1097/00004424-199011000-00006· OSTI ID:6322424

Papageorges, M; Gavin, P R; Adams, D S; Cheevers, W P; Barbee, D D; Sande, R D ^[1]

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1. Washington State Univ., Pullman (USA)

Caprine arthritis-encephalitis (CAE) is a model for the study of lentiviral infections. The authors' hypothesis is that radioimmunodetection has the potential to detect lentiviral proteins at the surface of infected cells. A monoclonal antibody (CAEV92A1) specific for a CAE virus (CAEV)-associated glycoprotein and a control antibody were radiolabeled with technetium-99m ({sup 99m}Tc) using the pretinning method. Cell binding assays were used to evaluate immunoreactivity and binding properties of {sup 99m}Tc-labeled antibodies to CAEV-infected cells. {sup 99m}Tc-CAEV92A1 bound preferentially to paraformaldehyde-fixed and live CAEV-infected cells. {sup 99m}Tc-CAEV92A1 did not appear to be shed rapidly from its binding site.

• OSTI ID: 6322424
• Journal Information: Investigative Radiology; (USA), Vol. 25:11; ISSN 0020-9996
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
MONOCLONAL ANTIBODIES
SPECIFICITY
VIRAL DISEASES
DIAGNOSIS
BIOCHEMICAL REACTION KINETICS
GLYCOPROTEINS
ISOMERIC NUCLEI
RADIOIMMUNOSCINTIGRAPHY
RECEPTORS
TECHNETIUM 99
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISEASES
HOURS LIVING RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
REACTION KINETICS
SCINTISCANNING
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics