Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co]

Leadon, S A; Ward, J F

doi:10.2307/3575461

Title: Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co]

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Abstract

A cell-free assay was developed to measure the effect of ..gamma..-irradiated DNA template on the ability of DNA polymerase to copy unirradiated template. Doses as low as 1 krad were able to decrease (approx. 15%) the activity of both bacterial and mammalian DNA polymerases in the assay. The percentage of polymerase activity decreased as the dose received by the template increased. The reduction in DNA polymerase activity was shown to be due to an inhibition of the enzyme by the irradiated DNA. Irradiated poly(dA-dT) was more effective in reducing polymerase activity than calf thymus DNA. Thus the polymerase-inhibition site(s) appears to be associated with base damage, specifically adenine or thymine. Using a free-radical scavenger, OH radicals were found to be involved in producing the damage sites. The interaction between irradiated DNA and DNA polymerase was found to be specific for the enzyme and not for other proteins present in the assay. The inhibition of DNA polymerase occurred prior to or during the initiation of DNA synthesis rather than after initiation of synthesis, i.e., during elongation.

Authors:: Leadon, S A; Ward, J F

Publication Date:: Mon Jun 01 00:00:00 EDT 1981

Research Org.:: Univ. of California, Los Angeles

OSTI Identifier:: 6318450

Resource Type:: Journal Article

Journal Name:: Radiat. Res.; (United States)

Additional Journal Information:: Journal Volume: 86:3

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL RADIATION EFFECTS; ENZYME ACTIVITY; INHIBITION; POLYMERASES; ADENINES; COBALT 60; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; HYDROXYL RADICALS; MICROCOCCUS LUTEUS; THYMINE; THYMUS; AMINES; ANTIMETABOLITES; AZINES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BODY; COBALT ISOTOPES; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LYMPHATIC SYSTEM; MICROCOCCUS; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHORUS-GROUP TRANSFERASES; PURINES; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADICALS; RADIOISOTOPES; TRANSFERASES; URACILS; YEARS LIVING RADIOISOTOPES; 560110* - Radiation Effects on Biochemicals- (-1987)

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                    Leadon, S A, and Ward, J F. Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co].  United States: N. p., 1981. 
        Web.  doi:10.2307/3575461.

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                    Leadon, S A, & Ward, J F. Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co].  United States.  https://doi.org/10.2307/3575461

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                    Leadon, S A, and Ward, J F. 1981.  
        "Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co]".  United States.  https://doi.org/10.2307/3575461.

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@article{osti_6318450,

  title        = {Effect of. gamma. -irradiated DNA on the activity of DNA polymerase. [/sup 60/Co]},

  author       = {Leadon, S A and Ward, J F},

  abstractNote = {A cell-free assay was developed to measure the effect of ..gamma..-irradiated DNA template on the ability of DNA polymerase to copy unirradiated template. Doses as low as 1 krad were able to decrease (approx. 15%) the activity of both bacterial and mammalian DNA polymerases in the assay. The percentage of polymerase activity decreased as the dose received by the template increased. The reduction in DNA polymerase activity was shown to be due to an inhibition of the enzyme by the irradiated DNA. Irradiated poly(dA-dT) was more effective in reducing polymerase activity than calf thymus DNA. Thus the polymerase-inhibition site(s) appears to be associated with base damage, specifically adenine or thymine. Using a free-radical scavenger, OH radicals were found to be involved in producing the damage sites. The interaction between irradiated DNA and DNA polymerase was found to be specific for the enzyme and not for other proteins present in the assay. The inhibition of DNA polymerase occurred prior to or during the initiation of DNA synthesis rather than after initiation of synthesis, i.e., during elongation.},

  doi          = {10.2307/3575461},

  url          = {https://www.osti.gov/biblio/6318450},
  journal      = {Radiat. Res.; (United States)},
number       = ,

  volume       = 86:3,

  place        = {United States},

  year         = {Mon Jun 01 00:00:00 EDT 1981},

  month        = {Mon Jun 01 00:00:00 EDT 1981}

}

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