New sensitive fluorometric method for measurement of vascular permeability
Journal Article
·
· J. Pharmacol. Methods; (United States)
A sensitive fluorometric method has been developed for the measurement of vascular permeability in carrageenin air-pouch inflammation in rats. Fluorescein isothiocyanate-labeled bovine serum albumin (F-BSA) was used as a tracer. This fluorometric method is as simple and reliable as the method using radioiodine-labeled human serum albumin and has the advantages of low cost, no health hazard, and the fact that F-BSA can be stored over a long period. This fluorometric method is probably applicable to other inflammation models such as pleurisy and peritonitis in which inflammatory exudate can be collected.
- Research Organization:
- Tohoku Univ., Sendai, Japan
- OSTI ID:
- 6306032
- Journal Information:
- J. Pharmacol. Methods; (United States), Vol. 11:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
LABELLING
IODINE ISOTOPES
COMPARATIVE EVALUATIONS
ISOTHIOCYANATES
BLOOD SERUM
CAPILLARIES
CATTLE
FLUORESCEIN
FLUORESCENCE SPECTROSCOPY
INFLAMMATION
MAN
MEASURING METHODS
PERMEABILITY
RATS
ANIMALS
AROMATICS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DOMESTIC ANIMALS
DYES
EMISSION SPECTROSCOPY
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
RODENTS
RUMINANTS
SPECTROSCOPY
SYMPTOMS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
ALBUMINS
LABELLING
IODINE ISOTOPES
COMPARATIVE EVALUATIONS
ISOTHIOCYANATES
BLOOD SERUM
CAPILLARIES
CATTLE
FLUORESCEIN
FLUORESCENCE SPECTROSCOPY
INFLAMMATION
MAN
MEASURING METHODS
PERMEABILITY
RATS
ANIMALS
AROMATICS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DOMESTIC ANIMALS
DYES
EMISSION SPECTROSCOPY
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
RODENTS
RUMINANTS
SPECTROSCOPY
SYMPTOMS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques