Studies involving the induction of prostaglandin synthesis following cell transformation by herpes simplex virus type 2

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6291842

Krebs, C R

The purpose of this study was to determine the effect of HSV-2 transformation on cellular metabolic processes, specifically the metabolism of arachidonic acid (20:4) and prostaglandin (PG) synthesis. Results obtained by labeling cells with (/sup 3/H)20:4 and analyzing the release of radioactivity into overlay culture medium demonstrate that while nontransformed rat embryo fibroblasts (REF) possess phospholipase to catalyze the release of 20:4 from membrane phospholipids, transformation of REF cells by photoinactivated HSV-2 virions induces cyclooxygenase to convert 20:4 substrate primarily to PGE/sub 2/ and PGF/sub 2..cap alpha../. Induction of 20:4 deacylation in nontransformed and HSV-2 transformed cells as well as PG synthesis in transformed cells is further enhanced by the tumor promoter (12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophore A23187. Phospholipase and cyclooxygenase appear to be coupled in their regulation in HSV-2 transformed tumor-derived rat fibrosarcoma (RFS) cells. Three times more (/sup 3/H)20:4 is incorporated into the phosphatidylserine/phosphatidylinositol (PS/PI) fraction in HSV-2 transformed cells compared to REF cells; additionally, this fraction serves as the primary donor of (/sup 3/H)20:4 released from TPA-stimulated transformed cells.

• Research Organization: Wake Forest Univ., Winston-Salem, NC (USA)
• OSTI ID: 6291842
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARACHIDONIC ACID
METABOLISM
FIBROBLASTS
CELL TRANSFORMATIONS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PROSTAGLANDINS
BIOSYNTHESIS
VIRUSES
EMBRYOS
HERPES SIMPLEX
LIPASE
OXYGENASES
PHOSPHOLIPIDS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DISEASES
ENZYMES
ESTERASES
ESTERS
HYDROLASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDOREDUCTASES
PARASITES
RODENTS
SKIN DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques
560300 - Chemicals Metabolism & Toxicology