Sensitivity of mitomycin C and nitrogen mustard crosslinks to extreme alkaline conditions
DNA-DNA crosslinks in cells treated with mitomycin C, nitrogen mustard, or decarbamoyl mitomycin C were measured in alkaline isopycnic gradients as a function of pH. Crosslinks from cells treated with mitomycin C and nitrogen mustard, which react with DNA purines, could be detected at pH 12.5 but not at pH 14. No crosslinks from cells treated with decarbamoyl mitomycin C were detected at either pH. Previous studies with cells exposed to psoralen derivatives plus 360 nm light, which produce DNA-DNA crosslinks with pyrimidines, demonstrated stable crosslinks at pH 14. These studies indicate that DNA-DNA crosslinks involving DNA purines are much less stable at high pH than those involving pyrimidines, and that methods involving exposure to extreme alkaline conditions may give inaccurate information for some agents. 25 references, 1 figure.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6291446
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 123:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MITOMYCIN
GENETIC EFFECTS
NITROGEN MUSTARD
CROSS-LINKING
DNA
METABOLIC ACTIVATION
PH VALUE
PURINES
PYRIMIDINES
ALKYLATING AGENTS
AMINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DRUGS
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)