Measurement of phthalates in small samples of mammalian tissue
Di-(2-ethylhexyl)-phthalate (DEHP) is a phthalic acid ester that is used as a plasticizer in polyvinyl chloride products, many of which have widespread medical application. DEHP has been shown to be leached from products used for storage and delivery of blood transfusions during procedures such as plasmaphoresis, hemodialysis and open heart surgery. Results of studies in this laboratory have suggested that there is an association between the absorption and deposition of DEHP (and/or related chemicals) in the kidney and the acquired renal cystic disease (ACD) frequently seen in patients who have undergone prolonged dialysis treatment. In order to determine the relationship between the two, it has been necessary to establish a method for extracting and accurately quantitating minute amounts of these chemicals in small tissue samples. The authors have now established such a method using kidneys from normal rats and from a rat model for ACD.
- Research Organization:
- Dalhousie Univ., Halifax, Nova Scotia
- OSTI ID:
- 6290436
- Journal Information:
- Bull. Environ. Contam. Toxicol.; (United States), Vol. 38:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
KIDNEYS
QUANTITATIVE CHEMICAL ANALYSIS
PHTHALATES
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
DIALYSIS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
PHARMACOLOGY
RATS
ANIMALS
BODY
CARBOXYLIC ACID SALTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANS
RODENTS
SEPARATION PROCESSES
SPECTROSCOPY
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550601 - Medicine- Unsealed Radionuclides in Diagnostics