Changes in GDP binding to brown adipose tissue mitochondria and the uncoupling protein

Journal Article · Thu Dec 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6289488

Swick, A G; Swick, R W ^[1]

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1. Univ. of Wisconsin, Madison (USA)

Incubation in vitro of brown adipose tissue (BAT) mitochondria with divalent cations, spermine, or alkaline phosphatase led to a marked increase in the binding of ({sup 3}H)GDP. The effect of Mg{sup 2+} appeared to be the most specific and led to the largest increase in GDP binding. A simplified method was developed for measuring GDP binding to purified uncoupling protein from rat BAT mitochondria. Application of this method indicates that uncoupling protein from cold-acclimated rats binds twice as much GDP as uncoupling protein from cold-acclimated rats that were briefly returned to thermoneutrality, paralleling changes in GDP binding to the mitochondria. Incubation of BAT mitochondria with Mg{sup 2+} led to a smaller increase in GDP binding to the subsequently purified uncoupling protein, suggesting that divalent cations may somehow participate in the regulation of the activity of the uncoupling protein.

• OSTI ID: 6289488
• Journal Information: American Journal of Physiology; (USA), Vol. 255:6; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ISOTOPE RATIO
CATIONS
BIOLOGICAL EFFECTS
NUCLEOTIDES
CROSS-LINKING
RECEPTORS
CHEMICAL ACTIVATION
TRITIUM COMPOUNDS
ADIPOSE TISSUE
ALKALINE PHOSPHATASE
CALCIUM COMPOUNDS
MAGNESIUM COMPOUNDS
MITOCHONDRIA
PROTEINS
SACCHAROSE
ALKALINE EARTH METAL COMPOUNDS
ANIMAL TISSUES
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL REACTIONS
CONNECTIVE TISSUE
DISACCHARIDES
ENZYMES
ESTERASES
HYDROGEN COMPOUNDS
HYDROLASES
IONS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHATASES
POLYMERIZATION
SACCHARIDES
TISSUES
551001* - Physiological Systems- Tracer Techniques