Effects of sodium on cell surface and intracellular TH-naloxone binding sites
The binding of the opiate antagonist TH-naloxone was examined in rat whole brain homogenates and in crude subcellular fractions of these homogenates (nuclear, synaptosomal, and mitochondrial fractions) using buffers that approximated intra- (low sodium concentration) and extracellular (high sodium concentration) fluids. Saturation studies showed a two-fold decrease in the dissociation constant (Kd) in all subcellular fractions examined in extracellular buffer compared to intracellular buffer. In contrast, there was no significant effect of the buffers on the Bmax. Thus, TH-naloxone did not distinguish between binding sites present on cell surface and intracellular tissues in these two buffers. These results show that the sodium effect of opiate antagonist binding is probably not a function of altered selection of intra- and extracellular binding sites. 17 references, 2 tables.
- Research Organization:
- Univ. of Virginia Medical Center, Charlottesville
- OSTI ID:
- 6286381
- Journal Information:
- Life Sci.; (United States), Vol. 41:4
- Country of Publication:
- United States
- Language:
- English
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