Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture
Bordetella pertussis synthesis a variety of virulence factors including a calmodulin-dependent adenylate cyclase (AC) toxin. Treatment of anterior pituitary cells with this AC toxin resulted in an increase in cellular cAMP levels that was associated with accelerated exocytosis of growth hormone (GH), prolactin, adrenocorticotropic hormone (ACTH), and luteinizing hormone (LH). The kinetics of release of these hormones, however, were markedly different; GH and prolactin were rapidly released, while LH and ACTH secretion was more gradually elevated. Neither dopamine agonists nor somatostatin changes the ability of AC toxin to generate cAMP (up to 2 h). Low concentrations of AC toxin amplified the secretory response to hypophysiotrophic hormones. The authors conclude that bacterial AC toxin can rapidly elevate cAMP levels in anterior pituitary cells and that it is the response that explains the subsequent acceleration of hormone release.
- Research Organization:
- Univ. of Virginia School of Medicine, Charlottesville
- OSTI ID:
- 6283311
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 251:2
- Country of Publication:
- United States
- Language:
- English
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ACTH
RADIOIMMUNOASSAY
SECRETION
CYCLASES
BIOLOGICAL EFFECTS
LH
LTH
STH
AMP
ANIMAL CELLS
BACTERIA
DOPAMINE
PITUITARY GLAND
RATS
TOXINS
AMINES
ANIMALS
ANTIGENS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ENDOCRINE GLANDS
ENZYMES
GLANDS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LYASES
MAMMALS
MATERIALS
MICROORGANISMS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOLS
PITUITARY HORMONES
POLYPHENOLS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SYMPATHOMIMETICS
TOXIC MATERIALS
TRACER TECHNIQUES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics