( sup 125 I)fibrin deposition occurs at both early and late intervals of IgE-dependent or contact sensitivity reactions elicited in mouse skin. Mast cell-dependent augmentation of fibrin deposition at early intervals in combined IgE-dependent and contact sensitivity reactions
- Harvard Medical School, Boston, MA (USA)
When elicited in the skin of mice, either IgE-dependent immediate hypersensitivity reactions or T cell-dependent contact sensitivity (CS) reactions result in local extravasation of (125I)fibrinogen and deposition of (125I)fibrin. However, these two types of reaction differ in kinetics and in requirement for IgE, mast cells, or T cells. In the present study, we investigated the kinetics and magnitude of (125I)fibrin deposition in combined IgE-dependent and CS reactions elicited simultaneously at the same site and compared the results with those obtained when the two reactions were elicited at separate sites. We found that (125I)fibrin deposition in pure IgE-dependent reactions was greater at 2 or 6 h after challenge than at 24 h, but that significant fibrin deposition persisted at those sites 24 h after challenge. In CS reactions, (125I)fibrin deposition was detected as early as 2 h after challenge, indicating that fibrin deposition accompanies the early component of CS detected by Van Loveren et al. with the use of measurements of tissue swelling. But much more (125I)fibrin deposition was present in CS reactions at 24 h than at 2 or 6 h after Ag challenge. When IgE-dependent and CS reactions were elicited at the same site, (125I)fibrin deposition at early intervals (2 to 6 h) after challenge was increased three- to 25-fold compared with that seen in isolated CS reactions, but at 24 h the results in the combined reactions were virtually identical to those in CS responses. Studies in genetically mast cell-deficient and congenic normal mice indicated that mast cells were required for expression of the IgE-dependent augmentation of (125I)fibrin deposition observed at early intervals in combined IgE-dependent and CS reactions, but not for the (125I)fibrin deposition associated with pure CS reactions.
- OSTI ID:
- 6275591
- Journal Information:
- Journal of Immunology; (USA), Vol. 145:11; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dissociation of cutaneous vascular permeability and the development of cutaneous late-phase allergic reactions
Suppression of IgE-mediated mast cell activation and mouse anaphylaxis via inhibition of Syk activation by 8-formyl-7-hydroxy-4-methylcoumarin, 4μ8C
Related Subjects
DERMATITIS
PATHOGENESIS
FIBRIN
BIOSYNTHESIS
IMMUNOGLOBULINS
IODINE 125
LYMPHOCYTES
MICE
SKIN
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COAGULANTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SCLEROPROTEINS
SKIN DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques