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Title: Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor

Abstract

A validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9 mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is greater by ten times or more than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by 30 cu m-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
OSTI Identifier:
6275358
Report Number(s):
PB-91-109231/XAB; EPA-600/J-88/542
Resource Type:
Technical Report
Resource Relation:
Other Information: Text in Japanese and English. Pub. in Jnl. Japan Soc. Air Pollut., Vol. 23, 24-31(1988). Portions of this document are not fully legible
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PARTICULATES; MUTAGEN SCREENING; INDOOR AIR POLLUTION; PARTICLE SIZE; SALMONELLA TYPHIMURIUM; SAMPLING; AIR POLLUTION; BACTERIA; MICROORGANISMS; PARTICLES; POLLUTION; SALMONELLA; SCREENING; SIZE; 540120* - Environment, Atmospheric- Chemicals Monitoring & Transport- (1990-); 560300 - Chemicals Metabolism & Toxicology

Citation Formats

Takagi, Y, Goto, S, Murata, M, Matsushita, H, and Lewtas, J. Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor. United States: N. p., 1988. Web.
Takagi, Y, Goto, S, Murata, M, Matsushita, H, & Lewtas, J. Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor. United States.
Takagi, Y, Goto, S, Murata, M, Matsushita, H, and Lewtas, J. 1988. "Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor". United States.
@article{osti_6275358,
title = {Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor},
author = {Takagi, Y and Goto, S and Murata, M and Matsushita, H and Lewtas, J},
abstractNote = {A validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9 mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is greater by ten times or more than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by 30 cu m-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample.},
doi = {},
url = {https://www.osti.gov/biblio/6275358}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}

Technical Report:
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