Synthesis of tritium labeled Ac-(Nle/sup 4/, D-Phe/sup 7/)-. cap alpha. -MSH/sub 4-11/-NH/sub 2/: a superpotent melanotropin with prolonged biological activity

Journal Article · Mon Mar 05 00:00:00 EST 1984 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(84)90302-3· OSTI ID:6271768

Wilkes, B D; Hruby, V J; Yamamura, H I; Akiyama, K; Castrucci, A.M. de; Hadley, M E; Andrews, J R; Wan, Y P

Ac-(Nle/sup 4/, D-Phe/sup 7/)-..cap alpha..-MSH/sub 4-11/-NH/sub 2/ an octapeptide, is a melanotropin analogue (Ac-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-NH/sub 2/), which is a superpotent agonist of frog and lizard skin melanocytes and mouse S 91 (Cloudman) melanoma cells. This melanotropin possesses ultraprolonged activity on melanocytes, both in vitro and in vivo, and the peptide is resistant to inactivation by serum enzymes. The tritium-labeled congener was prepared by direct incorporation of (/sup 3/H)-labeled norleucine into the peptide. The melanotropic activity of the labeled peptide is identical to the unlabeled analogue. This labeled peptide should be useful for studies on the localization and characterization of melanotropin receptors.

• Research Organization: Univ. of Arizona, Tucson
• OSTI ID: 6271768
• Journal Information: Life Sci.; (United States), Vol. 34:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
LABELLED COMPOUNDS
CHEMICAL PREPARATION
PITUITARY HORMONES
BIOSYNTHESIS
AMINO ACIDS
ANIMAL CELLS
BIOLOGICAL LOCALIZATION
FROGS
IN VITRO
IN VIVO
LIZARDS
MELANIN
MICE
RADIOASSAY
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PIGMENTS
REPTILES
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
400702 - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials