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Title: Synthesis of tritium labeled Ac-(Nle/sup 4/, D-Phe/sup 7/)-. cap alpha. -MSH/sub 4-11/-NH/sub 2/: a superpotent melanotropin with prolonged biological activity

Ac-(Nle/sup 4/, D-Phe/sup 7/)-..cap alpha..-MSH/sub 4-11/-NH/sub 2/ an octapeptide, is a melanotropin analogue (Ac-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-NH/sub 2/), which is a superpotent agonist of frog and lizard skin melanocytes and mouse S 91 (Cloudman) melanoma cells. This melanotropin possesses ultraprolonged activity on melanocytes, both in vitro and in vivo, and the peptide is resistant to inactivation by serum enzymes. The tritium-labeled congener was prepared by direct incorporation of (/sup 3/H)-labeled norleucine into the peptide. The melanotropic activity of the labeled peptide is identical to the unlabeled analogue. This labeled peptide should be useful for studies on the localization and characterization of melanotropin receptors.
Authors:
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Publication Date:
OSTI Identifier:
6271768
Resource Type:
Journal Article
Resource Relation:
Journal Name: Life Sci.; (United States); Journal Volume: 34:10
Research Org:
Univ. of Arizona, Tucson
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; LABELLED COMPOUNDS; CHEMICAL PREPARATION; PITUITARY HORMONES; BIOSYNTHESIS; AMINO ACIDS; ANIMAL CELLS; BIOLOGICAL LOCALIZATION; FROGS; IN VITRO; IN VIVO; LIZARDS; MELANIN; MICE; RADIOASSAY; RECEPTORS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMPHIBIANS; ANIMALS; AQUATIC ORGANISMS; CARBOXYLIC ACIDS; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDE HORMONES; PIGMENTS; REPTILES; RODENTS; SYNTHESIS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques; 400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials