skip to main content

Title: Radiosensitivity of human natural killer cells: Binding and cytotoxic activities of natural killer cell subsets

The sensitivity of human natural killer (NK) cell activities (both binding and killing) after exposure of peripheral blood mononuclear cells to different doses of gamma radiation was studied. A panel of monoclonal antibodies was used to identify the NK and T-lymphocyte subsets and to evaluate their radiosensitivity. Peripheral blood mononuclear cells were irradiated with low (2-6 Gy) and high (10-30 Gy) doses and NK cell binding and cytotoxic activity against K562 target cells were studied after 3 h and 48 h in culture. The primary damage to NK cell activity was identified at the postbinding level and affected mainly the lytic machinery. After 48 h culture postirradiation, an overall depression of cytotoxic activity was observed, but ionizing radiation produced either a selection of the more cytotoxic NK cell subsets, which therefore might be considered more resistant to radiation damage than the less cytotoxic NK cells, or a long-term stimulation of cytotoxic activity in surviving cells.
Authors:
; ; ; ;  [1]
  1. (Universita di Bologna (Italy))
Publication Date:
OSTI Identifier:
6271530
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiation Research; (USA); Journal Volume: 124:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; RADIOSENSITIVITY; MONOCYTES; BIOLOGICAL RADIATION EFFECTS; GAMMA RADIATION; MAN; MONOCLONAL ANTIBODIES; PHENOTYPE; RADIATION DOSES; ANIMAL CELLS; ANIMALS; ANTIBODIES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DOSES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; MAMMALS; MATERIALS; PRIMATES; RADIATION EFFECTS; RADIATIONS; SOMATIC CELLS; VERTEBRATES 560120* -- Radiation Effects on Biochemicals, Cells, & Tissue Culture