Dephosphorylation of WR-2721 with mouse tissue homogenates
Mouse liver homogenate had an optimum pH of 8.6 to 8.8 for dephosphorylation of WR-2721 in the analyzed pH range from 5.2 to 10.0. At this optimum pH condition, the dephosphorylation activities of six mouse tissue homogenates were analyzed. Kidney, liver and small intestine homogenates showed higher dephosphorylation activities than spleen and lung homogenates. Furthermore, serum did not show any dephosphorylation activity. The high activity found in liver homogenate agrees well with our previous data with mouse L cells. However, optimum pH from 8.6 to 8.8 in liver homogenate is quite different from the data reported by using Ehrlich ascites tumor cells (optimum pH was 5.6). Therefore, it is suggested that WR-2721 administered into mouse is efficiently dephosphorylated in certain tissues such as liver to its active form with the enzyme(s) different from that found in Ehrlich ascites tumor cells.
- Research Organization:
- Kanazawa Univ., Japan
- OSTI ID:
- 6264367
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 10:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIVER
HOMOGENATES
THIOLS
CHEMICAL REACTIONS
ASCITES TUMOR CELLS
BLOOD SERUM
KIDNEYS
LUNGS
MICE
PH VALUE
RADIOPROTECTIVE SUBSTANCES
SMALL INTESTINE
SPLEEN
ANIMAL CELLS
ANIMALS
BODY
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GLANDS
INTESTINES
MAMMALS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
TUMOR CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals