Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation

Hashimoto, K; Watanabe, Y; Uchida, S; Yoshida, H

doi:10.1016/0024-3205(89)90308-1

Title: Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation

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Abstract

After section of the sciatic nerve, the basal adenylate cyclase (AC) activity in rat gastrocnemius muscle increased 6-7 times per membrane protein and about 2 times per whole muscle in the following 30 or 40 days. The AC activity in the muscle 30 days after denervation was increased about 4 times by folskolin. Calcitonin gene-related peptide (CGRP) also increased the adenylate cyclase activity in the denervated muscle. The binding of (/sup 3/H)-forskolin to cells isolated from gastrocnemius muscle was examined to determine the amount of AC molecules. Inhibition of (/sup 3/H)-forskolin binding by increasing amounts of unlabeled forskolin gave a sigmoid curve with a IC/sub 50/ value of 3/times/10/sup /minus/7/M. Results showed that the number of (/sup 3/H)-forskolin binding sites per cell was higher on the denervated side than on the control side, like the basal AC activity. The IC/sub 50/ values for inhibition by unlabeled forskolin of binding of (/sup 3/H)-forskolin were similar to muscles on the control and denervated sides. These results suggest that an increase in the AC activity induced by denervation was due to an increase in the numbers of AC molecules in the muscle.

Authors:: Hashimoto, K; Watanabe, Y; Uchida, S; Yoshida, H

Publication Date:: Sun Jan 01 00:00:00 EST 1989

Research Org.:: Osaka Univ. Medical School (Japan)

OSTI Identifier:: 6257683

Resource Type:: Journal Article

Journal Name:: Life Sci.; (United States)

Additional Journal Information:: Journal Volume: 44:24

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; CYCLASES; ENZYME ACTIVITY; MUSCLES; BIOCHEMISTRY; PHOSPHOLIPIDS; BIOCHEMICAL REACTION KINETICS; MEMBRANE PROTEINS; NERVES; PEPTIDES; RATS; RECEPTORS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; CHEMISTRY; ENZYMES; ESTERS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; LIPIDS; LYASES; MAMMALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PROTEINS; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

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                    Hashimoto, K, Watanabe, Y, Uchida, S, and Yoshida, H. Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation.  United States: N. p., 1989. 
        Web.  doi:10.1016/0024-3205(89)90308-1.

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                    Hashimoto, K, Watanabe, Y, Uchida, S, & Yoshida, H. Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation.  United States.  https://doi.org/10.1016/0024-3205(89)90308-1

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                    Hashimoto, K, Watanabe, Y, Uchida, S, and Yoshida, H. 1989.  
        "Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation".  United States.  https://doi.org/10.1016/0024-3205(89)90308-1.

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@article{osti_6257683,

  title        = {Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation},

  author       = {Hashimoto, K and Watanabe, Y and Uchida, S and Yoshida, H},

  abstractNote = {After section of the sciatic nerve, the basal adenylate cyclase (AC) activity in rat gastrocnemius muscle increased 6-7 times per membrane protein and about 2 times per whole muscle in the following 30 or 40 days. The AC activity in the muscle 30 days after denervation was increased about 4 times by folskolin. Calcitonin gene-related peptide (CGRP) also increased the adenylate cyclase activity in the denervated muscle. The binding of (/sup 3/H)-forskolin to cells isolated from gastrocnemius muscle was examined to determine the amount of AC molecules. Inhibition of (/sup 3/H)-forskolin binding by increasing amounts of unlabeled forskolin gave a sigmoid curve with a IC/sub 50/ value of 3/times/10/sup /minus/7/M. Results showed that the number of (/sup 3/H)-forskolin binding sites per cell was higher on the denervated side than on the control side, like the basal AC activity. The IC/sub 50/ values for inhibition by unlabeled forskolin of binding of (/sup 3/H)-forskolin were similar to muscles on the control and denervated sides. These results suggest that an increase in the AC activity induced by denervation was due to an increase in the numbers of AC molecules in the muscle.},

  doi          = {10.1016/0024-3205(89)90308-1},

  url          = {https://www.osti.gov/biblio/6257683},
  journal      = {Life Sci.; (United States)},
number       = ,

  volume       = 44:24,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 1989},

  month        = {Sun Jan 01 00:00:00 EST 1989}

}

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