Water permeability of primary mouse keratinocyte cultures grown at the air-liquid interface

Journal Article · Sat Apr 01 00:00:00 EST 1989 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12712043· OSTI ID:6257074

Cumpstone, M B; Kennedy, A H; Harmon, C S; Potts, R O

In order to study the development of the epidermal permeability barrier in vitro, tritiated water (HTO) flux was measured across murine keratinocytes cultured at the air-liquid interface. Using a micro-diffusion technique, it was shown that air-liquid cultures form areas where the water diffusion is comparable to that of intact neonatal mouse skin. When water permeability is measured over a large area of the culture surface, however, significantly higher flux is obtained. These results show that under the culture conditions used, areas of water barrier comparable to intact neonatal mouse skin coexist with regions of less complete barrier formation.

• Research Organization: Pfizer Central Research, Groton, CT (USA)
• OSTI ID: 6257074
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 92:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PERMEABILITY
WATER
MEMBRANE TRANSPORT
ANIMAL CELLS
CELL CULTURES
CYTOLOGICAL TECHNIQUES
EPIDERMIS
KERATIN
NEONATES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL TISSUES
BODY
CELL CONSTITUENTS
EPITHELIUM
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
SCLEROPROTEINS
SKIN
TISSUES
550301* - Cytology- Tracer Techniques