Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses
Plaques formed by herpes simplex virus (HSV), pseudorabies virus, and varicella-zoster virus were studied by plaque autoradiography after (/sup 14/C)thymidine labeling. Standard thymidine kinase-positive (TK+) viruses and TK- mutants of HSV types 1 and 2 and pseudorabies virus were studied, including cell cultured viruses and viruses isolated from animals. Autoradiography was performed with X-ray film with an exposure time of 5 days. After development of films, TK+ plaques showed dark rims due to isotope incorporation, whereas TK- plaques were minimally labeled. Plaque autoradiography of stock TK- viruses showed reversion frequencies to the TK+ phenotype of less than 10(-3). Autoradiography indicated that TK- virus retained the TK- phenotype after replication in vivo. In addition, it was shown that TK- HSV could be isolated from mouse trigeminal ganglion tissue after corneal inoculation of TK- HSV together with TK+ HSV. The plaque autoradiographic procedure was very useful to evaluate proportions of TK+ and TK- virus present in TK+-TK- virus mixtures.
- Research Organization:
- Department of Medicine, Pennsylvania State University College of Medicine, Hershey
- OSTI ID:
- 6257026
- Journal Information:
- J. Clin. Microbiol.; (United States), Vol. 17:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HERPES SIMPLEX
PLAQUE FORMATION
HERPES ZOSTER
THYMIDINE
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
PHOSPHOTRANSFERASES
AZINES
DISEASES
ENZYMES
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LABELLED COMPOUNDS
NERVOUS SYSTEM DISEASES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PYRIMIDINES
RIBOSIDES
SKIN DISEASES
TRANSFERASES
VIRAL DISEASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics