Membranes replace irradiated blast cells as growth requirement for leukemic blast progenitors in suspension culture

Journal Article · Fri Nov 01 00:00:00 EST 1985 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.162.5.1435· OSTI ID:6255571

Nara, N; McCulloch, E A

The blast cells of acute myeloblastic leukemia (AML) may be considered as a renewal population, maintained by blast stem cells capable of both self-renewal and the generation of progeny with reduced or absent proliferative potential. This growth requires that two conditions be met: first, the cultures must contain growth factors in media conditioned either by phytohemagglutinin (PHA)-stimulated mononuclear leukocytes (PHA-LCM), or by cells of the continuous bladder carcinoma line HTB9 (HTB9-CM). Second, the cell density must be maintained at 10(6) blasts/ml; this may be achieved by adding irradiated cells to smaller numbers of intact blasts. The authors are concerned with the mechanism of the feeding function. They present evidence that (a) cell-cell contact is required. (b) Blasts are heterogeneous in respect to their capacity to support growth. (c) Fractions containing membranes from blast cells will substitute for intact cells in promoting the generation of new blast progenitors in culture. (d) This membrane function may be specific for AML blasts, since membranes from blasts of lymphoblastic leukemia or normal marrow cells were inactive.

• Research Organization: Ontario Cancer Institute, Toronto, Canada
• OSTI ID: 6255571
• Journal Information: J. Exp. Med.; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
CELL DIVISION
CELL MEMBRANES
LEUKEMIA
TUMOR CELLS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DISEASES
HEMIC DISEASES
LEUKOCYTES
MATERIALS
MEMBRANES
NEOPLASMS
RADIATION EFFECTS
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)