Effect of prior immobilization on muscular glucose clearance in resting and running rats

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6252817

Vissing, J; Ohkuwa, Tetsuo; Ploug, T; Galbo, H ^[1]

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1. Univ. of Copenhagen (Denmark) Nagoya Institute of Technology (Japan)

In vitro studies have shown that prior disuse impairs the glucose clearance of red skeletal muscle because of a developed insensitivity to insulin. We studied whether an impaired glucose clearance is present in vivo in 42-h immobilized muscles of resting rats and, furthermore, whether the exercise-induced increase in glucose clearance of red muscles is affected by prior immobilization. The 2-({sup 3}H)deoxy-D-glucose (2DG) bolus injection method was used to determine glucose clearance of individual muscles. At rest, glucose clearance was markedly impaired in rats with previously immobilized red muscles compared with nonimmobilized control rats. During running, glucose clearance did not differ between muscles in previously immobilized and control rats. Insulin levels were always similar in the two groups and decreased during exercise. Intracellular nonphosphorylated 2DG was present in tissues with high glucose clearances. In conclusion, 42 h of immobilization markedly impairs glucose clearance of resting red muscle fibers in vivo. Apparently, physical inactivity in particular affects steps involved in insulin-mediated action that are not part of contraction-induced glucose uptake and metabolism. Presence of intracellular 2DG shows that separate determination of phosphorylated 2DG is necessary for accurate estimates of glucose metabolism and that accumulation of phosphorylated 2DG does not accurately reflect glucose transport.

• OSTI ID: 6252817
• Journal Information: American Journal of Physiology; (USA), Vol. 255:4; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
BLOOD-PLASMA CLEARANCE
MUSCLES
PHYSIOLOGY
TRITIUM COMPOUNDS
TRACER TECHNIQUES
EXERCISE
GLYCOGEN
INSULIN
LACTIC ACID
NORADRENALINE
PHOSPHORYLATION
RATS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
CLEARANCE
DRUGS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
MAMMALS
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
POLYSACCHARIDES
RODENTS
SACCHARIDES
SYMPATHOMIMETICS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques