The incorporation of myo-inositol into phosphatidylinositol derivatives is stimulated during hormone-induced meiotic maturation of amphibian oocytes

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(90)90020-B· OSTI ID:6250604

Carrasco, D; Allende, C C; Allende, J E ^[1]

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1. Universidad de Chile, Casilla, Santiago (Chile)

The incorporation of myo-({sup 3}H)inositol into phosphatidylinositol and its phosphorylated derivatives was studied by microinjection of the radioactive precursor into Xenopus laevis oocytes. Induction of meiotic maturation of the oocytes by treatment with either progesterone one or insulin resulted in a significant increase in the incorporation of myo-({sup 3}H)inositol into the phospholipid fraction. This increase occurred 3-6 h after hormonal treatment, a time coincident with the start of the breakdown of the nuclear envelope, and requires protein synthesis. The effect of progesterone and insulin contrasts with the effect of acetylcholine, which acts through a muscarinic receptor causing the activation of phospholipase C, since the latter effector causes an increase in myo-({sup 3}H)inositol incorporation, which is more rapid and does not require protein synthesis. These results suggest that the meiotic maturation process is connected with changes in inositol metabolism in the amphibian oocyte.

• OSTI ID: 6250604
• Journal Information: Experimental Cell Research; (USA), Vol. 191:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INOSITOL
METABOLISM
LIPASES
ENZYME ACTIVITY
OOCYTES
MITOSIS
PHOSPHOLIPIDS
BIOSYNTHESIS
ACETYLCHOLINE
FROGS
INSULIN
PROGESTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOHYDRATES
CARBOXYLESTERASES
CELL DIVISION
DRUGS
ENZYMES
ESTERASES
ESTERS
GERM CELLS
HORMONES
HYDROGEN COMPOUNDS
HYDROLASES
INOSITOLS
ISOTOPE APPLICATIONS
KETONES
LIPIDS
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDE HORMONES
PREGNANES
QUATERNARY COMPOUNDS
SACCHARIDES
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques