Rapid changes in deoxynucleoside triphosphate pools in mammalian cells treated with mutagens

Journal Article · Fri Jul 29 00:00:00 EDT 1983 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(83)90802-1· OSTI ID:6250297

Das, S K; Benditt, E P; Loeb, L A

The rapid increase in cellular deoxynucleoside triphosphate (dNTP) concentrations in Chinese Hamster cell line V79 after exposure to known mutagens is described. With this cell line an expansion of dATP and dTTP pools was detected; changes in dCTP were not large; changes in dGTP were either not significant or too low to quantitate. This situation may reflect the existence of imbalances in dNTP pools at the DNA replication fork. The expansion of dATP and dTTP pools occurred within 2 to 4 hours after exposure of cultured cells to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Ultraviolet light (UV), mitomycin C, and cytosine arabinoside also caused similar dNTP pool changes.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6250297
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 114:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GUANIDINES
MUTAGENESIS
NITROSO COMPOUNDS
ULTRAVIOLET RADIATION
BIOCHEMICAL REACTION KINETICS
CHO CELLS
CYTOSINE
MITOMYCIN
AMINES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
CARBONIC ACID DERIVATIVES
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
KINETICS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
REACTION KINETICS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)